Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Rodovia Ilhéus-Itabuna, km 16, CEP 45650-900, Ilhéus, BA, Brazil.
J Invertebr Pathol. 2013 Feb;112(2):138-41. doi: 10.1016/j.jip.2012.11.003. Epub 2012 Nov 29.
This study investigated the occurrence of the protozoan Perkinsus in the oyster Crassostrea rhizophorae on the coast of Bahia State, Brazil. The oysters (n = 900) were collected in February-March and July-August 2010. The Ray's fluid thioglycollate medium (RFTM) analysis of gills and rectum revealed hypnospores of Perkinsus sp. with a high mean prevalence (63%). The infection intensity varied from very light to advanced. The polymerase chain reaction confirmed Perkinsus in 87.2% of the RFTM-positive oysters. Histological analysis showed trophozoites and schizonts phagocytized by hemocytes, mainly in the intestine and the stomach epithelium.
本研究调查了巴西巴伊亚州沿海地区牡蛎 Crassostrea rhizophorae 中原生动物 Perkinsus 的发生情况。2010 年 2 月至 3 月和 7 月至 8 月期间采集了这些牡蛎(n = 900)。对鳃和直肠的 Ray 液体巯基醋酸盐培养基(RFTM)分析显示,存在高平均流行率(63%)的 Perkinsus sp. 的休眠孢子。感染强度从轻度到重度不等。聚合酶链反应证实,在 87.2%的 RFTM 阳性牡蛎中存在 Perkinsus。组织学分析显示,血细胞吞噬了滋养体和裂殖体,主要在肠和胃上皮中。