基于石墨烯和多功能酶修饰碳纳米球的电化学免疫传感器用于微囊藻毒素-LR 的检测。

A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection.

机构信息

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

出版信息

Colloids Surf B Biointerfaces. 2013 Mar 1;103:38-44. doi: 10.1016/j.colsurfb.2012.10.010. Epub 2012 Oct 13.

DOI:10.1016/j.colsurfb.2012.10.010

PMID:23201717

Abstract

A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase-carbon nanosphere-antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15 μgL(-1) microcystin-LR with a detection limit of 0.016 μgL(-1). Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins.

摘要

一种基于石墨烯的免疫传感器被制备出来，用于检测微囊藻毒素-LR，使用辣根过氧化物酶-碳纳米球-抗体系统进行信号放大。石墨烯和壳聚糖被用作固定化材料，以提高电极的电化学性能。使用多酶功能化的碳纳米球实现了信号放大。该方法提供了一个从 0.05 到 15 μgL(-1) 的微囊藻毒素-LR 的线性范围，检测限为 0.016 μgL(-1)。通过比较免疫传感器和高效液相色谱法得到的结果，证明了该电化学免疫传感器在环境水样中对微囊藻毒素-LR 的分析检测能力。这种电化学免疫传感器具有高灵敏度、良好的稳定性、可重复性，有望用于各种毒素的常规水质监测。