Department of Pharmacy, School of Pharmacy, University of Oslo, PO Box 1068 Blindern, 0316 Oslo, Norway.
Colloids Surf B Biointerfaces. 2013 Mar 1;103:158-65. doi: 10.1016/j.colsurfb.2012.10.012. Epub 2012 Oct 13.
Pectin is a polymer with well-known mucoadhesive properties. In this study, liposomes were coated with three different types of pectin. Their properties were characterized and their mucoadhesiveness was estimated by a novel in vitro approach. Two different types of commercially available mucin were investigated in order to choose the best candidate for the method. The effect of pH on the properties of the coated liposomes and the interaction with mucin was also studied. The pectin-coated liposomes and the complexes they formed with mucin were characterized by dynamic light scattering (DLS), zeta potential and turbidity measurements. The zeta potential of the liposomes shifted from positive to negative after coating with pectin. They also exhibited larger diameters, and the liposomes coated with HM-pectin were the largest. After the addition of mucin, the zeta potential shifted to a less negative value and the sizes of the pectin-coated liposomes increased. The complexes formed between mucin and the HM-pectin-coated liposomes were the largest, while the smallest were formed with the LM-pectin-coated liposomes. The pH was found to affect the interaction between the coated liposomes and mucin. DLS was conducted on an ALV goniometer to gain information about the diffusivity of the samples, the relative scattered intensities and to obtain an optimal characterization of the size distributions. The results correlated well with measurements from an automatized light scattering instrument (Zetasizer Nano ZS).
果胶是一种具有良好黏附性能的聚合物。在这项研究中，脂质体被三种不同类型的果胶所包裹。通过一种新的体外方法对其特性进行了表征，并对其黏附性能进行了评估。为了选择该方法的最佳候选物，研究了两种不同类型的市售黏蛋白。还研究了 pH 值对包裹脂质体性质以及与黏蛋白相互作用的影响。通过动态光散射（DLS）、zeta 电位和浊度测量对果胶包裹的脂质体及其与黏蛋白形成的复合物进行了表征。果胶包裹脂质体的 zeta 电位从正变为负。它们的直径也更大，其中 HM-pectin 包裹的脂质体最大。加入黏蛋白后，zeta 电位移向更负的值，并且果胶包裹的脂质体的尺寸增加。在 HM-pectin 包裹的脂质体和黏蛋白之间形成的复合物最大，而 LM-pectin 包裹的脂质体形成的复合物最小。发现 pH 值影响包裹脂质体和黏蛋白之间的相互作用。在 ALV 折射计上进行 DLS 以获取有关样品扩散率、相对散射强度的信息，并对尺寸分布进行最佳表征。结果与自动光散射仪（Zetasizer Nano ZS）的测量结果很好地相关。
