Department of Pharmacy, School of Pharmacy, University of Oslo, Oslo, Norway.
Colloids Surf B Biointerfaces. 2011 Dec 1;88(2):664-73. doi: 10.1016/j.colsurfb.2011.07.058. Epub 2011 Aug 6.
The present study investigated the surface coating of charged liposomes by three different types of pectin (LM, HM and amidated pectin) by particle size determinations and zeta potential measurements. The pectins and the pectin coated liposomes were visualized by atomic force microscopy. The adsorption of pectin onto positive liposomes yielded a reproducible increase in particle size and a shift of the zeta potential from positive to negative side for all three pectin types, whereas the adsorption of pectin onto negative liposomes did not render any significant changes probably due to electrostatic repulsion. The positive liposomes coated with HM-pectin gave the largest pectin coated particles with the least negative zeta potential, while the opposite was observed for the LM-pectin coated positive liposomes. Furthermore, results from dynamic light scattering revealed narrow size distributions, indicating that the degree of aggregation was low for the pectin coated liposomes. As liposomes are able to encapsulate drugs and pectin has been found to be mucoadhesive, these pectin coated liposomes may be potential drug delivery systems.
本研究通过粒径测定和zeta 电位测量，考察了三种不同类型的果胶（LM、HM 和酰胺化果胶）对带电荷脂质体的表面包覆作用。通过原子力显微镜对果胶和包被有果胶的脂质体进行了可视化观察。果胶吸附到正电脂质体上，导致颗粒尺寸重现性增加，zeta 电位从正移到负，所有三种果胶类型均如此，而果胶吸附到负电脂质体上则没有明显变化，可能是由于静电排斥。带正电的脂质体用 HM-果胶包被后得到的带正电的果胶包被粒子最大，zeta 电位最小，而 LM-果胶包被的带正电的脂质体则相反。此外，动态光散射的结果显示出较窄的粒径分布，表明果胶包被的脂质体的聚集程度较低。由于脂质体能够包封药物，而果胶已被发现具有粘膜粘附性，因此这些包被有果胶的脂质体可能是潜在的药物递送系统。
