School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Colloids Surf B Biointerfaces. 2013 Mar 1;103:209-16. doi: 10.1016/j.colsurfb.2012.10.018. Epub 2012 Oct 17.
In this study, calcium phosphate cement (CPC)-based scaffold with unidirectional lamellar pore structure was fabricated by unidirectional freeze casting. Poly(lactic-co-glycolic acid) (PLGA) was infiltrated into the CPC scaffold to improve its strength and toughness, which compromised the bioactivity and osteoconductivity of CPC. Collagen (Col) was immobilized on the pore surface of the PLGA/CPC scaffold to enhance the bioactivity of the scaffold using plasma treatment under the ammonia (NH(3)) atmosphere. The immobilization of collagen was characterized by infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Compared to the PLGA/CPC composite scaffold, the Col/PLGA/CPC composite scaffold had higher contact angle, porosity and water absorption, while the compressive strength of both scaffolds was comparable. Rat bone marrow mesenchymal stem cells (rMSCs) seeded on the Col/PLGA/CPC scaffold showed markedly improved cell seeding, attachment, proliferation and differentiation than those on the PLGA/CPC scaffold. These results suggest that the surface immobilization of collagen by plasma treatment can improve the bioactivity of the PLGA/CPC scaffold and the Col/PLGA/CPC composite scaffold is a promising candidate for bone tissue engineering.
在这项研究中，通过单向冷冻铸造制备了具有各向异性层状孔结构的磷酸钙水泥（CPC）支架。聚（乳酸-共-乙醇酸）（PLGA）被渗透到 CPC 支架中以提高其强度和韧性，这会影响 CPC 的生物活性和骨传导性。使用氨（NH（3））气氛下的等离子体处理将胶原蛋白（Col）固定在 PLGA/CPC 支架的孔表面上，以增强支架的生物活性。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）和扫描电子显微镜（SEM）对胶原蛋白的固定进行了表征。与 PLGA/CPC 复合材料支架相比，Col/PLGA/CPC 复合支架具有更高的接触角、孔隙率和吸水率，而两种支架的抗压强度相当。在 Col/PLGA/CPC 支架上接种的大鼠骨髓间充质干细胞（rMSCs）的细胞接种、附着、增殖和分化明显优于在 PLGA/CPC 支架上的细胞。这些结果表明，等离子体处理表面固定胶原蛋白可以提高 PLGA/CPC 支架的生物活性，Col/PLGA/CPC 复合支架是骨组织工程的有前途的候选材料。
