Yang Yongbo, Abdalla Soliman
Department of Orthopedics (Spine), Xinxiang Central Hospital, Xinxiang City, China.
Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Front Bioeng Biotechnol. 2020 Oct 15;8:951. doi: 10.3389/fbioe.2020.00951. eCollection 2020.
Skeletal defects resulting from trauma and disease represent a major clinical problem worldwide exacerbated further by global population growth and an increasing number of elderly people. As treatment options are limited, bone tissue engineering opens the doors to start an infinite amount of tissue/bone biomaterials having excellent therapeutic potential for the management of clinical cases characterized by severe bone loss. Bone engineering relies on the use of compliant biomaterial scaffolds, osteocompetent cells, and biologically active agents. In fact, we are interested to use a new natural material, tannin. Among other materials, porous tannin spray-dried powder (PTSDP) has been approved for human use. We use PTSDP as reconstructive materials with low cost, biocompatibility, and potential ability to be replaced by bone . In this study, macro PTSDP scaffolds with defined geometry, porosity, and mechanical properties are manufactured using a combination of casting technology and porogen leaching, by mixing PTSDP and hydroxyapatite Ca10(PO4)6(OH)2 with polyethylene glycol macroparticles. Our results show that the scaffolds developed in this work support attachment, long-term viability, and osteogenic differentiation of human-induced pluripotent stem cell-derived mesenchymal progenitors. The combination of select macroporous PTSDP scaffolds with patient-specific osteocompetent cells offers new opportunities to grow autologous bone grafts with enhanced clinical potential for complex skeletal reconstructions.
由创伤和疾病导致的骨骼缺陷是全球范围内的一个重大临床问题，全球人口增长和老年人口数量增加使这一问题进一步恶化。由于治疗选择有限，骨组织工程为开发大量具有卓越治疗潜力的组织/骨生物材料打开了大门，这些材料可用于治疗以严重骨丢失为特征的临床病例。骨工程依赖于使用顺应性生物材料支架、具有成骨能力的细胞和生物活性剂。事实上，我们对使用一种新型天然材料——单宁感兴趣。在其他材料中，多孔单宁喷雾干燥粉末（PTSDP）已被批准用于人类。我们将PTSDP用作具有低成本、生物相容性且有被骨替代的潜在能力的重建材料。在本研究中，通过将PTSDP与羟基磷灰石Ca10(PO4)6(OH)2和聚乙二醇大颗粒混合，利用铸造技术和致孔剂浸出相结合的方法，制造出具有特定几何形状、孔隙率和机械性能的宏观PTSDP支架。我们的结果表明，这项工作中开发的支架支持人诱导多能干细胞来源的间充质祖细胞的附着、长期存活和成骨分化。选择的大孔PTSDP支架与患者特异性的具有成骨能力的细胞相结合，为生长具有增强临床潜力的自体骨移植物提供了新机会，可用于复杂的骨骼重建。
