Koprivová H, Novák F, Poucková P, Svihovcová P, Jantscheff P, Bötgger V, Micheel B, Dvorák P, Seblová J, Pospísil J
Institute of Biophysics and Nuclear Medicine, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
Neoplasma. 1990;37(1):3-11.
Monoclonal antibody D11-DG2 (DG2) against carcinoembryonic antigen (CEA) was examined for suitability for radioimmunodetection of human tumors grown in nude mice. Antibodies DG2 and a control antibody of the same IgG1 subclass were labeled with 131I and injected into mice bearing one of three types of CEA-containing tumors (cell lines LS 174T, HT-29 and Rec S) and/or a CEA-negative tumor (Rec R). Gamma-camera imaging and distribution studies revealed that CEA-containing tumors selectively accumulate DG2 but Rec R does not. As the tumors differ in CEA-content, the highest accumulation of 131I-DG2 (corresponding to the best scintigraphic imaging) was found in LS 174T tumors, intermediate in Rec S and lowest in HT-29 tumors. The mean tumor-to-blood ratios on the sixth day after antibody administration were 4.6, 3.2, and 2.1, respectively, in the control experiments the value of this parameter was always lower than 1. The results showed the applicability of DG2 for immunoscintigraphic studies in patients. Furthermore, a positive correlation was found between the uptake of anti-CEA antibody and CEA-content in the tumors.
检测了抗癌胚抗原（CEA）的单克隆抗体D11-DG2（DG2）对裸鼠体内生长的人类肿瘤进行放射免疫检测的适用性。将抗体DG2和相同IgG1亚类的对照抗体用131I标记，然后注射到携带三种含CEA肿瘤（细胞系LS 174T、HT-29和Rec S）之一和/或CEA阴性肿瘤（Rec R）的小鼠体内。γ相机成像和分布研究表明，含CEA的肿瘤选择性地积聚DG2，但Rec R不积聚。由于肿瘤的CEA含量不同，在LS 174T肿瘤中发现131I-DG2的积聚最高（对应最佳的闪烁成像），在Rec S中居中，在HT-29肿瘤中最低。抗体给药后第六天的平均肿瘤与血液比值分别为4.6、3.2和2.1，在对照实验中该参数的值始终低于1。结果表明DG2适用于患者的免疫闪烁研究。此外，还发现抗CEA抗体的摄取与肿瘤中的CEA含量呈正相关。
