College of Life Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Bioresour Technol. 2013 Jan;128:526-32. doi: 10.1016/j.biortech.2012.10.107. Epub 2012 Nov 2.
HD-1, a stable microbial consortium capable of mineralizing di-n-butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25-30 °C and pH 8.0-9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography-mass spectrometry (GC-MS) during the degradation process. The HD-1 consortium also utilized phenol, o-dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation.
从活性污泥中开发出一种能够矿化邻苯二甲酸二丁酯（DBP）的稳定微生物联合体 HD-1。通过变性梯度凝胶电泳（DGGE）鉴定出联合体中的优势微生物为戈登氏菌、伯克霍尔德氏菌和无色杆菌。在培养 48 小时后，联合体可以矿化约 1200mg/L DBP 的 90%。DBP 降解的最佳条件为 25-30°C 和 pH 8.0-9.0。添加酵母（0.5g/L）、乙酸钠（0.5g/L、1.0g/L）、Brij 35（0.2%、0.5%、1.0%）或 Triton X-100（0.2%）可增强 DBP 的降解。邻苯二甲酸二甲酯（DMP）和邻苯二甲酸二乙酯（DEP）的存在会影响 DBP 的降解速率。在降解过程中，仅通过气相色谱-质谱联用仪（GC-MS）可以监测到一种主要的中间产物邻苯二甲酸。HD-1 联合体还利用苯酚、邻苯二酚作为唯一的碳源和能源。结果表明，该联合体可能代表了 DBP 生物修复的一种很有前途的应用。
