改良胆胰分流术对 GK 大鼠葡萄糖耐量的影响。

Effects of modified biliopancreatic diversion on glucose tolerance of GK rats.

机构信息

Hepatopancreatobiliary Surgery Department, The First Affiliated Hospital of Fujian Medical University, Cha-Zhong Road, Fuzhou, 350005, People's Republic of China.

出版信息

Obes Surg. 2013 Apr;23(4):522-30. doi: 10.1007/s11695-012-0830-x.

DOI:10.1007/s11695-012-0830-x

PMID:23207830

Abstract

BACKGROUND

The purpose of this study was to investigate the hypoglycemic effect of new biliopancreatic diversion and duodenal-jejunal bypass in Goto-Kakizaki rats and observe effects of the new surgical procedure on the glucose tolerance of GK rats.

METHODS

Twenty-four 10-week-old rats (SPF grade) were randomly divided into groups A, B, and C, each with eight rats. Group A underwent duodenal-jejunal bypass, group B underwent modified biliopancreatic diversion, and group C underwent a sham operation. Median rat body weight, fasting blood glucose, OGTT, and blood lipids were measured in fasting 1 week before surgery and 1, 2, 4, and 8 weeks after surgery. Changes in gastric inhibitory polypeptide, glucagon P-like peptide-1, and insulin levels were measured by ELISA 1 week before surgery and 8 weeks after surgery.

RESULTS

Rats' mean body weight in groups A and B decreased significantly from 368.025 ± 11.726 and 373.100 ± 9.859 g preoperatively to 345.750 ± 11.403 and 343.260 ± 12.399 g at the early postoperative stage (P < 0.05), and with statistically significant differences compared to the weight of rats in group C (P < 0.05). Comparisons between fasting blood glucose before surgery and 8 weeks after surgery revealed no significant differences between all three groups (P > 0.05). Glucose tolerance in groups A and B decreased from preoperative 21.175 ± 3.684 and 20.820 ± 1.671 mmol/L to postoperative 8.950 ± 0.580 and 10.500 ± 1.509 mmol/L, and both were better than that of group C (P < 0.001).

CONCLUSIONS

Both new biliopancreatic diversion and duodenal-jejunal bypass improve glucose tolerance of Goto-Kakizaki rats.

摘要

背景

本研究旨在探讨新型胆胰分流和十二指肠空肠旁路术对 Goto-Kakizaki 大鼠的降糖作用，并观察新手术方式对 GK 大鼠葡萄糖耐量的影响。

方法

将 24 只 10 周龄 SPF 级大鼠随机分为 A、B、C 三组，每组 8 只。A 组行十二指肠空肠旁路术，B 组行改良胆胰分流术，C 组行假手术。术前 1 周及术后 1、2、4、8 周测量大鼠中位体质量、空腹血糖、OGTT 和血脂，术前 1 周及术后 8 周采用 ELISA 法检测胃抑制肽、胰高血糖素样肽-1 和胰岛素水平的变化。

结果

A、B 两组大鼠术前体质量分别为 368.025 ± 11.726、373.100 ± 9.859 g，术后早期分别降至 345.750 ± 11.403、343.260 ± 12.399 g，与 C 组比较差异均有统计学意义（P < 0.05）。三组大鼠术前空腹血糖比较差异无统计学意义（P > 0.05），术后 8 周与术前比较差异均无统计学意义（P > 0.05）。A、B 两组大鼠葡萄糖耐量较术前均下降，由术前 21.175 ± 3.684、20.820 ± 1.671 mmol/L 下降至术后 8.950 ± 0.580、10.500 ± 1.509 mmol/L，均优于 C 组（P < 0.001）。