趋化因子基质细胞衍生因子 1 和单核细胞趋化因子 1 可促进人神经祖细胞向白细胞介素-1β的定向迁移。

Directed migration of human neural progenitor cells to interleukin-1β is promoted by chemokines stromal cell-derived factor-1 and monocyte chemotactic factor-1 in mouse brains.

机构信息

Department of Pharmacology and Experimental Neuroscience, Neuroimmunology and Regenerative Therapy Laboratory, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA.

出版信息

Transl Neurodegener. 2012 Jul 31;1(1):15. doi: 10.1186/2047-9158-1-15.

BACKGROUND

Neurogenesis, including the proliferation, migration and differentiation of neural progenitor cells (NPCs), is impaired in HIV-1 associated dementia (HAD). We previously demonstrated HIV-1-infected macrophages (HIV-MDM) regulate stromal cell-derived factor 1 (SDF-1) production in astrocytes through Interleukin-1β (IL-1β). Chemokines are known to induce NPC migration; however, it remains unclear how chemokines produced in inflammation regulate NPC migration.

METHODS

The secretion of SDF-1 and Monocyte chemotactic preotein-1 (MCP-1) in astrocytes upon IL-1β stimulation was measured by ELISA assay. Human NPCs were injected parallel along with IL-1β, SDF-1 or MCP-1 intracranially into basal ganglion 1 mm apart in SCID mice, and immunofluorescent staining was used to study the survival and migration of injected human NPCs.

RESULTS

SDF-1 and MCP-1 are secreted by astrocytes upon IL-1β stimulation in a time-dependent manner. Injected human NPCs survived in SCID mice and migrated towards sites of IL-1β, SDF-1 and MCP-1 injection.

CONCLUSIONS

In conclusion, chemokines SDF-1 or MCP-1 secreted by astrocytes in the presence of IL-1β injection are attractive to NPCs injected into SCID mouse brains, suggesting that SDF-1 and MCP-1 play important roles in NPC migration during neuroinflammation.

背景

HIV-1 相关性痴呆（HAD）患者的神经发生受到损害，包括神经祖细胞（NPC）的增殖、迁移和分化。我们之前的研究表明，HIV-1 感染的巨噬细胞（HIV-MDM）通过白细胞介素 1β（IL-1β）调节星形胶质细胞中基质细胞衍生因子 1（SDF-1）的产生。趋化因子已知可诱导 NPC 迁移；然而，炎症中产生的趋化因子如何调节 NPC 迁移仍不清楚。