Cassiano Nm, Barreiro Jc, Oliveira Rv, Cass Qb
Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, 13565-905 São Carlos - SP, Brazil.
Bioanalysis. 2012 Nov;4(22):2737-56. doi: 10.4155/bio.12.226.
New analytical platforms have been developed in response to the need for attaining increased peak capacity for multicomponent complex analysis with higher sensitivity and characterization of the analytes, and high-throughput capabilities. This review outlines the fundamental principles of target and comprehensive 2D LC method development and encompasses applications of LC-LC and LC × LC coupled to MS in bioanalysis using a variety of online analytical procedures. It also provides a rationale for the usage of the most employed mass analyzers and ionization sources on these platforms.
为满足多组分复杂分析中提高峰容量、实现更高灵敏度和分析物表征以及高通量能力的需求，已开发出新型分析平台。本综述概述了目标二维液相色谱法和全二维液相色谱法方法开发的基本原理，并涵盖了液相色谱-液相色谱联用和液相色谱×液相色谱联用质谱在生物分析中的应用，采用了多种在线分析程序。还阐述了在这些平台上使用最常用的质量分析器和离子源的基本原理。
