Department of Civil and Environmental Engineering, Virginia Tech, 415 Durham Hall, Blacksburg, Virginia 24061, United States.
Anal Chem. 2013 Feb 5;85(3):1342-9. doi: 10.1021/ac301028w. Epub 2013 Jan 9.
A honeycomb-shaped gold substrate was developed for surface-enhanced Raman imaging (SERI). The honeycombs are composed of clusters of 50-70 nm gold nanoparticles and exhibit high Raman enhancement efficiency. An average surface enhancement factor (ASEF) of 1.7 × 10(6) was estimated for a monolayer of L-cysteine molecules adsorbed to gold via a thiol linkage. The presence of a linear relationship in the low concentration region was observed in SERI detection of malachite green isothiocyanate (MGITC). These results together with the high reproducibility and simple and cost-effective fabrication of this substrate suggest that it has utility for applications of surface-enhanced Raman scattering (SERS) in quantitative diagnoses and analyte detection.
一种蜂窝状金基底被开发用于表面增强拉曼成像（SERI）。这些蜂窝由 50-70nm 金纳米粒子簇组成，具有高的拉曼增强效率。通过硫醇键吸附到金上的 L-半胱氨酸单层的平均表面增强因子（ASEF）估计为 1.7×10(6)。在 SERI 检测孔雀石绿异硫氰酸盐（MGITC）的低浓度区域观察到线性关系的存在。这些结果以及该基底具有的高重现性、简单且具有成本效益的制造工艺表明，它可用于表面增强拉曼散射（SERS）在定量诊断和分析物检测中的应用。
