State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
J Integr Plant Biol. 2013 May;55(5):473-83. doi: 10.1111/jipb.12021. Epub 2013 Feb 4.
A stable inherited rice spotted-leaf mutant HM47 derived from an EMS-induced IR64 mutant bank was identified. The mutant expressed hypersensitive response (HR)-like symptoms throughout its whole life from the first leaf to the flag leaf, without pathogen invasion. Initiation of the lesions was induced by light under natural summer field conditions. Expression of pathogenesis-related genes including PAL, PO-C1, POX22.3 and PBZ1 was enhanced significantly in association with cell death and accumulation of H2 O2 at and around the site of lesions in the mutant in contrast to that in the wild-type (WT). Disease reaction to Xanthomonas oryzae pv. oryzae from the Philippines and China showed that HM47 is a broad-spectrum disease-resistant mutant with enhanced resistance to multiple races of bacterial blight pathogens tested. An F2 progeny test showed that bacterial blight resistance to race HB-17 was co-segregated with the expression of lesions. Genetic analysis indicated that the spotted-leaf trait was controlled by a single recessive gene, tentatively named spl(HM47) , flanked by two insertion/deletion markers in a region of approximately 74 kb on the long arm of chromosome 4. Ten open reading frames are predicted, and all of them are expressed proteins. Isolation and validation of the putative genes are currently underway.
从 EMS 诱导的 IR64 突变体库中鉴定出一个稳定遗传的水稻斑点叶突变体 HM47。该突变体从第一片叶到旗叶，在整个生命周期中都表现出类似于过敏反应（HR）的症状，而没有病原体的入侵。在自然夏季田间条件下，光照会引发病变的起始。与野生型（WT）相比，与细胞死亡和病变部位及其周围 H2O2 积累相关的病程相关基因（包括 PAL、PO-C1、POX22.3 和 PBZ1）的表达显著增强。对菲律宾和中国的稻黄单胞菌 pv.oryzae 的抗病反应表明，HM47 是一个广谱抗病突变体，对多种测试的细菌性条斑病病原体具有增强的抗性。F2 后代测试表明，对 HB-17 菌系的细菌性条斑病抗性与病变的表达共分离。遗传分析表明，斑点叶性状由一个单隐性基因控制，暂定名为 spl(HM47)，位于第 4 号染色体长臂约 74kb 的区域内，由两个插入/缺失标记侧翼。预测有十个开放阅读框，它们都是表达蛋白。目前正在进行候选基因的分离和验证。
