State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
J Integr Plant Biol. 2011 Aug;53(8):671-81. doi: 10.1111/j.1744-7909.2011.01056.x. Epub 2011 Jul 24.
A rice spotted-leaf mutant was isolated from an ethane methyl sulfonate (EMS) -induced IR64 mutant bank. The mutant, designated as spl30 (spotted-leaf30), displayed normal green leaf color under shade but exhibited red-brown lesions under natural summer field conditions. Initiation of the lesions was induced by light and the symptom was enhanced at 33 (°) C relative to 26 (°) C. Histochemical staining did not show cell death around the red-brown lesions. Chlorophyll contents in the mutant were significantly lower than those of the wild type while the ratio of chlorophyll a/b remained the same, indicating that spl30 was impaired in biosynthesis or degradation of chlorophyll. Disease reaction patterns of the mutant to Xanthomonas oryzae pv. oryzae were largely unchanged to most races tested except for a few strains. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively named spl30(t), which co-segregated with RM15380 on chromosome 3, and was delimited to a 94 kb region between RM15380 and RM15383. Spl30(t) is likely a novel rice spotted-leaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided in this study will enable further fine-mapping and cloning of the gene.
从乙烷甲基磺酸（EMS）诱导的 IR64 突变体库中分离出一个水稻黄斑突变体。该突变体被命名为 spl30（黄斑 30），在遮荫下表现出正常的绿叶颜色，但在自然夏季田间条件下表现出红棕色病斑。病斑的起始是由光照诱导的，与 26°C 相比，在 33°C 时症状增强。组织化学染色显示红棕色病斑周围没有细胞死亡。突变体中的叶绿素含量明显低于野生型，而叶绿素 a/b 的比值保持不变，表明 spl30 在叶绿素的生物合成或降解中受到损害。除了少数菌株外，突变体对稻黄单胞菌 pv. 稻种的反应模式与大多数测试的菌株基本相同。遗传分析表明，突变由一个单隐性基因控制，暂命名为 spl30(t)，该基因与第 3 号染色体上的 RM15380 共分离，并被限定在 RM15380 和 RM15383 之间的 94 kb 区域内。由于在该染色体区域附近没有发现其他类似的基因，因此 spl30(t) 很可能是一个新的水稻黄斑基因。本研究提供的遗传数据和重组群体将有助于进一步精细定位和克隆该基因。
