U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
J Food Prot. 2012 Dec;75(12):2234-7. doi: 10.4315/0362-028X.JFP-12-234.
We evaluated the effects of storage and handling conditions on the antimicrobial activity of biodegradable composite films (polylactic acid and sugar beet pulp) coated with allyl isothiocyanate (AIT). Polylactic acid and chitosan were incorporated with AIT and used to coat one side of the film. The films were subjected to different storage conditions (storage time, storage temperature, and packed or unpacked) and handling conditions (washing, abrasion, and air blowing), and the antimicrobial activity of the films against Salmonella Stanley in tryptic soy broth was determined. The films (8.16 μl of AIT per cm(2) of surface area) significantly (P < 0.05) inhibited the growth of Salmonella during 24 h of incubation at 22°C, while the populations of Salmonella in controls increased from ca. 4 to over 8 log CFU/ml, indicating a minimum inactivation of 4 log CFU/ml on films in comparison to the growth on controls. Statistical analyses indicated that storage time, storage temperature, and surface abrasion affected the antimicrobial activity of the films significantly (P < 0.05). However, the differences in microbial reduction between those conditions were less than 0.5 log cycle. The results suggest that the films' antimicrobial properties are stable under practical storage and handling conditions and that these antimicrobial films have potential applications in food packaging.
我们评估了储存和处理条件对包被丙烯基异硫氰酸酯（AIT）的可生物降解复合膜（聚乳酸和糖甜菜浆）的抗菌活性的影响。将聚乳酸和壳聚糖与 AIT 混合并用于膜的一侧涂覆。将膜置于不同的储存条件（储存时间、储存温度、包装或未包装）和处理条件（洗涤、磨损和吹气）下，并测定膜对胰蛋白酶大豆肉汤中的斯坦利沙门氏菌的抗菌活性。在 22°C 孵育 24 小时期间，包被 AIT 的膜（每平方厘米表面积 8.16 μl 的 AIT）显著（P<0.05）抑制了沙门氏菌的生长，而对照中的沙门氏菌数量从约 4 增加到超过 8 对数 CFU/ml，表明与对照相比，膜上的最小失活为 4 对数 CFU/ml。统计分析表明，储存时间、储存温度和表面磨损显著影响膜的抗菌活性（P<0.05）。然而，这些条件下微生物减少的差异小于 0.5 对数循环。结果表明，在实际储存和处理条件下，膜的抗菌性能稳定，这些抗菌膜在食品包装中有潜在的应用。
