Honda Shigeru, Bessho Hiroaki, Kondo Naoshi, Kusuhara Sentaro, Tsukahara Yasutomo, Negi Akira
Department of Surgery, Division of Ophthalmology, Kobe University Graduate School of Medicine, Kobe, Japan.
Mol Vis. 2012;18:2796-804. Epub 2012 Nov 22.
To clarify the association between cluster of differentiation 36 (CD36) gene polymorphisms and the response to photodynamic therapy (PDT) in polypoidal choroidal vasculopathy (PCV).
One hundred and thirty-seven patients with PCV were enrolled. The patients were treated with PDT and followed up for more than 6 months. Retreatments were performed every 3 months as needed based on findings from angiography. Patients who showed an improvement in their best-corrected visual acuity at 6 months post-PDT were classified as PDT responders, and the others were defined as non-responders. For the 73 responders and 64 non-responders, 19 single nucleotide polymorphisms (SNPs) across the CD36 region were genotyped using the TaqMan assay. We analyzed the association between these variants and the visual outcomes of PDT.
The allelic frequencies of the SNPs rs3211851, rs3173798, and rs3211908 showed nominally significant differences between the PDT responders and non-responders. Genotype association analysis revealed a significant association of SNP rs3173798 with the visual outcome of PDT in a dominant model. The presence of the C allele in rs3173798 was significantly associated with a poor response to PDT after multivariate logistic regression analysis with clinical pre-PDT parameters. The mean best-corrected visual acuity in the group with the TT genotype of rs3173798 was significantly improved over 12 months of follow-up after the initial PDT.
The coding variants in CD36 are possibly associated with the visual outcome of PDT in patients with PCV.
阐明分化簇36（CD36）基因多态性与息肉状脉络膜血管病变（PCV）光动力疗法（PDT）疗效之间的关联。
纳入137例PCV患者。患者接受PDT治疗并随访6个月以上。根据血管造影结果，必要时每3个月进行一次再次治疗。PDT治疗后6个月最佳矫正视力有所改善的患者被分类为PDT反应者，其他患者则被定义为无反应者。对于73例反应者和64例无反应者，使用TaqMan分析对CD36区域的19个单核苷酸多态性（SNP）进行基因分型。我们分析了这些变异与PDT视觉结果之间的关联。
SNP rs3211851、rs3173798和rs3211908的等位基因频率在PDT反应者和无反应者之间显示出名义上的显著差异。基因型关联分析显示，在显性模型中，SNP rs3173798与PDT的视觉结果存在显著关联。在对PDT前临床参数进行多因素逻辑回归分析后，rs3173798中C等位基因的存在与PDT反应不佳显著相关。rs3173798的TT基因型组在初始PDT后的12个月随访中，平均最佳矫正视力有显著改善。
CD36中的编码变异可能与PCV患者PDT的视觉结果相关。
