Li Xiangting, Cai Depei
Research Department of Integrated Traditional and Western Medicine, Children's Hospital of Fudan University, Shanghai 200032, China.
Wei Sheng Yan Jiu. 2012 Sep;41(5):710-6.
To investigate the separate and combined toxic effects of di-2-ethylhexylphthalate (DEHP) and cypermethrin (CYP) on prepubertal male rats.
A total of 24 healthy 3-week-old SPF male Sprague-Dawley rats were randomly divided into four groups. They were control group (fed with corn oil), DEHP group (500 mg/kg, dissolved in corn oil), CYP group (80 mg/kg, dissolved in corn oil), combine group (DEHP 500 mg/kg + CYP 80 mg/kg, dissolved in corn oil), respectively. Each group consists of 6 rats. All the rats were exposed to DEHP or CYP by the means of gavage administration, once a day for 30 days. They were executed 24 hours after the last administration, and their weight, wet weight of the testis and testis coefficient was calculated. The histomorphological examination of the testis was conducted and the serum testosterone level was measured by Radioimmunoassay. The activities of LDH, ACP, ALP and SDH were assessed in testis homogenate.
Compared with control group, the time of testicular descent and preputial separation was significantly delayed and the anogenital distance was significantly shorter in DEHP group, CYP group and combine group (P < 0.05 or 0.01). In DEHP group, the wet weight of testis, testis coefficient and the serum level of testosterone were significantly decreased (P < 0.05). The activity of ALP, ACP and LDH in testis homogenate was significantly increased, but that of SDH was significantly decreased (P < 0.01). Histologic structure and ultrastructure of testis showed obvious abnormalities in DEHP group, CYP group and combine group.
DEHP and CYP had obviously separate and combined reproductive toxicity to prepubertal male rats. Both of them could cause the degeneration, necrosis and dysfunction of the seminiferous epithelium, sertoli cells and leydig cells and resulted in the abnormal development and function of the male reproductive system. Rats exposed to DEHP alone showed main toxic effect. There was no interactive influence on reproductive toxicity between DEHP and CYP in the combine group.
探讨邻苯二甲酸二（2-乙基己基）酯（DEHP）与氯氰菊酯（CYP）对青春期前雄性大鼠的单独及联合毒性作用。
将24只健康的3周龄SPF级雄性Sprague-Dawley大鼠随机分为四组。分别为对照组（喂饲玉米油）、DEHP组（500mg/kg，溶于玉米油）、CYP组（80mg/kg，溶于玉米油）、联合组（DEHP 500mg/kg + CYP 80mg/kg，溶于玉米油）。每组6只大鼠。所有大鼠均通过灌胃给予DEHP或CYP，每天1次，共30天。末次给药后24小时处死大鼠，计算其体重、睾丸湿重及睾丸系数。进行睾丸组织形态学检查，采用放射免疫法测定血清睾酮水平。检测睾丸匀浆中乳酸脱氢酶（LDH）、酸性磷酸酶（ACP）、碱性磷酸酶（ALP）及琥珀酸脱氢酶（SDH）的活性。
与对照组相比，DEHP组、CYP组及联合组睾丸下降和包皮分离时间明显延迟，肛殖距明显缩短（P < 0.05或0.01）。DEHP组睾丸湿重、睾丸系数及血清睾酮水平明显降低（P < 0.05）。睾丸匀浆中ALP、ACP及LDH活性明显升高，而SDH活性明显降低（P < 0.01）。DEHP组、CYP组及联合组睾丸组织学结构和超微结构均显示明显异常。
DEHP和CYP对青春期前雄性大鼠具有明显的单独及联合生殖毒性。二者均可导致生精上皮、支持细胞及间质细胞的变性、坏死及功能障碍，进而引起雄性生殖系统发育及功能异常。单独暴露于DEHP的大鼠毒性作用为主。联合组中DEHP与CYP在生殖毒性方面无交互影响。
