Zhou Zhirong, Wang Jun, Lin Shaobin
Institute for Environmental Health and Related Product Safety, Chinese CDC, Beijing 100021, China.
Wei Sheng Yan Jiu. 2012 Sep;41(5):853-7.
To establish the method to determine the presence and concentration of 8 organophosphors pesticides in serum by LC/MS/MS.
The serum samples were extracted by ethyl acetateether and then separated on C18 5 microm (2.1 mm x 150 mm) column with pH = 3.5 acid-ammonium acetate buffer solution, methanol--water as mobile phase to mixed gradient elution. Electro-spray ionization source (ESI) was applied and operated in positive ion mode, and multiple reaction monitoring(MRM) mode was adopted.
Within concentration ranging from 0-1000 ng/ml the correlation coefficient for each compound was greater than 0.990, recoveries were between 95%-129%, RSD < 7.9%. The RSD of intra-day and inter-day were less 12% (n = 6).
The procedure of preparing serum samples is sample and the result of this method is efficient, accurate and specific.
建立液相色谱-串联质谱法测定血清中8种有机磷农药的方法。
血清样品用乙酸乙酯-乙醚萃取，然后在pH = 3.5的乙酸铵缓冲溶液、甲醇-水为流动相的C18 5微米（2.1毫米×150毫米）柱上进行分离，采用混合梯度洗脱。采用电喷雾电离源（ESI），以正离子模式运行，并采用多反应监测（MRM）模式。
在0-1000纳克/毫升的浓度范围内，各化合物的相关系数均大于0.990，回收率在95%-129%之间，相对标准偏差（RSD）<7.9%。日内和日间RSD均小于12%（n = 6）。
血清样品制备过程简单，该方法结果高效、准确、特异。
