Du Zhong-bo, Mao Rui-ming, Gao Wei-min, Mi Li, Cao Zhi-peng, Zhu Bao-li
School of Forensic Medicine, China Medical University, Shenyang 110001, China.
Fa Yi Xue Za Zhi. 2012 Oct;28(5):327-32.
To observe the changes of hypoxia-inducible factor-1 alpha (HIF-1alpha), the expression in the early stage (within 6 h) of acute myocardial ischemia and to explore the potential forensic application.
SD rats were randomly divided into one control group, one sham operation group and five myocardial ischemia groups which received ligation of the left anterior descending (LAD) coronary artery. The five experiment groups divided into 15min, 30min, 1 h, 3 h and 6h after LAD ligation. The expression of HIF-1alpha was detected by immunohistochemistry, immunofluorescence and Western blotting, respectively.
Both the control group and sham operation group showed no expression of HIF-1alpha, whereas the expression of HIF-1alpha could be weakly detected beneath the endocardium at 15 min after LAD ligation. With the increase of myocardial ischemia process, the positive staining gradually extended from endocardium to epicardium, reached the peak at 3 h, and began to decrease gradually at 6h after LAD ligation but still maintained at a relatively high level. In addition, the expression of HIF-1alpha without a time-dependent way was also detected in full thickness of the right ventricle in occurrence of ventricular arrhythmia after LAD ligation.
HIF-1alpha may be regarded as a sensitive marker for sudden cardiac death induced by early acute myocardial ischemia, and may also be helpful for the diagnosis of fatal arrhythmia.
观察缺氧诱导因子-1α（HIF-1α）在急性心肌缺血早期（6小时内）的表达变化，并探讨其潜在的法医学应用价值。
将SD大鼠随机分为1个对照组、1个假手术组和5个心肌缺血组，对心肌缺血组进行左冠状动脉前降支（LAD）结扎。5个实验组分别于LAD结扎后15分钟、30分钟、1小时、3小时和6小时取材。分别采用免疫组织化学、免疫荧光和蛋白质印迹法检测HIF-1α的表达。
对照组和假手术组均未检测到HIF-1α的表达，而在LAD结扎后15分钟可在心内膜下微弱检测到HIF-1α的表达。随着心肌缺血时间的延长，阳性染色逐渐从心内膜向心外膜扩展，在LAD结扎后3小时达到高峰，6小时后开始逐渐下降，但仍维持在较高水平。此外，在LAD结扎后发生室性心律失常时，右心室全层也检测到HIF-1α的表达，且无时间依赖性。
HIF-1α可作为早期急性心肌缺血所致心源性猝死的敏感标志物，也有助于致命性心律失常的诊断。
