[调查员HDplex试剂盒在中国东部汉族人群中的法医学应用]
[Forensic application of investigator HDplex kit in Han nationality of Eastern China].
作者信息
Zhang Su-hua, Zhang Zhe-ren, Sun Kuan, Zhao Zhen-min, Li Cheng-tao
机构信息
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China.
出版信息
Fa Yi Xue Za Zhi. 2012 Oct;28(5):351-4.
OBJECTIVE
To investigate the genetic data of 12 autosomal STR loci included in Investigator HDplex kit and to evaluate its forensic application in Han nationality of Eastern China.
METHODS
A total of 484 unrelated healthy individuals in Han nationality of Eastern China were investigated with Investigator HDplex kit. Allele frequencies, population genetics parameters and linkage disequilibrium information of the 12 autosomal STR loci were statistically analyzed.
RESULTS
No deviations from Hardy-Weinberg equilibrium were detected and all loci were independent form each other within the studied 484 unrelated healthy individuals. DP values of the 12 autosomal STR loci were all above 0.8, and CDP was 0.999 999 999 92. The cumulative probability of paternity exclusion in duo and in trio were 0.999 82 and 0.999 998 6, respectively.
CONCLUSION
Investigator HDplex kit with 12 highly polymorphic STR loci in Han nationality of Eastern China could be used effectively for forensic DNA genotyping.
目的
研究Investigator HDplex试剂盒中12个常染色体STR基因座的遗传数据，并评估其在中国东部汉族人群中的法医学应用价值。
方法
采用Investigator HDplex试剂盒对484名中国东部汉族无关健康个体进行调查。对12个常染色体STR基因座的等位基因频率、群体遗传学参数及连锁不平衡信息进行统计学分析。
结果
在484名无关健康个体中，未检测到偏离Hardy-Weinberg平衡的情况，且所有基因座相互独立。12个常染色体STR基因座的DP值均大于0.8，累积个体识别率为0.999 999 999 92。二联体和三联体的累积非父排除率分别为0.999 82和0.999 998 6。
结论
Investigator HDplex试剂盒中的12个高度多态性STR基因座在中国东部汉族人群中可有效用于法医DNA基因分型。
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