Wang Xiu-jin, Li Zhao-sheng, Xing Guan-lan, Li Zhuo-ning, Yuan Hong-li, Yang Jin-shui
State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Huan Jing Ke Xue. 2012 Aug;33(8):2735-40.
To improve the biomass and lipid productivity of the microalgae Chlorella pyrenoidosa-15, the carbon and nitrogen sources were screened to culture it heterotrophically. The best carbon and nitrogen sources were glucose and soy peptone, respectively. The carbon and nitrogen concentrations were optimized with the help of response surface design. The maximum biomass productivity was predicted to be 0.62 g x (L x d)(-1) with glucose and soy peptone concentrations of 17.53 g x L(-1) and 8.67 g x L(-1), respectively. The results of response surface design were validated with biomass productivity of 0.63 g x (L x d)(-1) and lipid content of 19.25%. The lipid productivity reached 121.3 mg x (L x d)(-1). In the research of Chlorella pyrenoidosa-15 cultured in non-autoclaved Beijing urban wastewater, the maximum algae biomass dry weight of 1.00 g x L(-1) was achieved with a lipid content of 24.12%. Results also showed that the treatment using Chlorella pyrenoidosa-15 effectively reduced the COD values and total nitrogen content in the wastewater, with a COD degradation rate of 80.9%, and a 69% decrease in total nitrogen content.
为提高小球藻Chlorella pyrenoidosa - 15的生物量和脂质生产率，筛选了碳源和氮源以进行异养培养。最佳碳源和氮源分别为葡萄糖和大豆蛋白胨。借助响应面设计对碳氮浓度进行了优化。预测葡萄糖和大豆蛋白胨浓度分别为17.53 g·L⁻¹和8.67 g·L⁻¹时，最大生物量生产率为0.62 g·(L·d)⁻¹。响应面设计结果经验证，生物量生产率为0.63 g·(L·d)⁻¹，脂质含量为19.25%。脂质生产率达到121.3 mg·(L·d)⁻¹。在对小球藻Chlorella pyrenoidosa - 15在未灭菌的北京城市污水中培养的研究中，实现了最大藻类生物量干重1.00 g·L⁻¹，脂质含量为24.12%。结果还表明，使用小球藻Chlorella pyrenoidosa - 15处理有效地降低了废水中的COD值和总氮含量，COD降解率为80.9%，总氮含量降低了69%。
