Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
Prep Biochem Biotechnol. 2013;43(1):22-32. doi: 10.1080/10826068.2012.693897.
A newly isolated strain Streptomyces sp. BG5 was investigated for the production of bioactive compounds. The strain exhibited broad-spectrum activity against an array of nine test organisms including gram-positive bacteria, gram-negative bacteria, and fungal and microalgal pathogens, along with a moderate cytotoxic response (28.9% mortality) in a microwell cytotoxicity assay against the brine shrimp Artimia salina. The morphological, physiological, and biochemical characterization of the Streptomyces sp. BG5 strongly suggested it to be a member of the genus Streptomyces. The nucleotide sequence of 16S rRNA gene (1433 pb) of the Streptomyces sp. BG5 (Gene bank accession number EU301836) exhibited high similarity (98%) with Streptomyces matensis. The large-scale fermentation of Streptomyces sp. BG5 and subsequent extraction, isolation, and purification of the crude extract afforded three pure compounds. The structures of these compounds were identified as ochromycinone (1a), emycin D (2), and 1-acetyl-β-carbolin (3), based on nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and by comparison with reference data from the literature.
一株新分离的链霉菌 sp. BG5 被研究用于生产生物活性化合物。该菌株对包括革兰氏阳性菌、革兰氏阴性菌以及真菌和微藻病原体在内的九种测试生物具有广谱活性，并且在针对卤虫 Artemia salina 的微量细胞毒性测定中具有中度细胞毒性反应（28.9%的死亡率）。链霉菌 sp. BG5 的形态学、生理学和生物化学特征强烈表明它是链霉菌属的一员。链霉菌 sp. BG5 的 16S rRNA 基因（1433 pb）（基因银行登录号 EU301836）的核苷酸序列与 Streptomyces matensis 表现出高度相似性（98%）。对链霉菌 sp. BG5 的大规模发酵以及随后的粗提物的提取、分离和纯化提供了三种纯化合物。这些化合物的结构通过核磁共振（NMR）光谱、质谱和与文献中的参考数据进行比较被鉴定为 ochromycinone（1a）、emycin D（2）和 1-acetyl-β-carbolin（3）。
