Department of Ophthalmology, Duzce University Medical Faculty, Duzce, Turkey.
J Ocul Pharmacol Ther. 2013 May;29(4):427-30. doi: 10.1089/jop.2012.0075. Epub 2012 Dec 6.
The aim of this study was to investigate the neuroprotective effect of olanzapine (OLA), an atypical antipsychotic drug, on N-methyl-d-aspartate (NMDA)-induced retinal injury.
Retinal neuronal ischemia was induced by NMDA in Wistar rats. OLA was administered intraperitoneally in 2 different dosages: 2 and 12 mg/kg. At the end of 2 weeks of OLA treatment, 1 eye of each animal was enucleated for histopathologic examination. We also measured malondialdehyde (MDA) levels in retinal homogenates as a marker of ischemic injury.
The retinal ganglion cell (RGC) count was significantly higher in cases where we used OLA 2 mg/kg or OLA 12 mg/kg compared to the control group (P=0.0032 and P=0.0005, respectively). We also found that MDA was significantly reduced by OLA 2 mg/kg or OLA 12 mg/kg compared to the control group (P=0.0001 and P=0.0001, respectively). There was no statistically significant difference between OLA 2 mg/kg or OLA 12 mg/kg groups in terms of RGC count and MDA levels (P>0.05 for all).
Our data showed that OLA preserved RGCs from NMDA-induced retinal injury; thus, it may have potential neuroprotective effects.
本研究旨在探讨新型抗精神病药物奥氮平（OLA）对 N-甲基-D-天冬氨酸（NMDA）诱导的视网膜损伤的神经保护作用。
通过 NMDA 在 Wistar 大鼠中诱导视网膜神经元缺血。OLA 通过腹腔内注射以 2 种不同剂量给药：2 和 12mg/kg。在 OLA 治疗 2 周结束时，每组动物的 1 只眼被摘出用于组织病理学检查。我们还测量了视网膜匀浆中的丙二醛（MDA）水平作为缺血损伤的标志物。
与对照组相比，使用 OLA 2mg/kg 或 OLA 12mg/kg 的情况下，视网膜神经节细胞（RGC）计数明显更高（P=0.0032 和 P=0.0005）。我们还发现，与对照组相比，OLA 2mg/kg 或 OLA 12mg/kg 显著降低了 MDA（P=0.0001 和 P=0.0001）。OLA 2mg/kg 或 OLA 12mg/kg 组之间在 RGC 计数和 MDA 水平方面没有统计学上的显著差异（P>0.05）。
我们的数据表明，OLA 可保护 RGC 免受 NMDA 诱导的视网膜损伤；因此，它可能具有潜在的神经保护作用。
