生物活性哈维肉瘤病毒未整合线性DNA的物理图谱。
Physical map of biologically active Harvey sarcoma virus unintegrated linear DNA.
作者信息
Goldfarb M P, Weinberg R A
出版信息
J Virol. 1979 Oct;32(1):30-9. doi: 10.1128/JVI.32.1.30-39.1979.
BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair linear DNA induced focal transformation upon transfection of NIH 3T3 mouse fibroblasts, and the biological activity of HSV DNA did not require helper MuLV functions. A physical map of restriction endonuclease cleavage sites along HSV 6.0-kilobase pair linear DNA was derived. Comparison of this map with one for Moloney MuLV DNA showed that the HSV and Moloney MuLV genomes are identical near their viral RNA 3' ends.
最近感染了哈维肉瘤病毒-鼠白血病病毒（HSV-MuLV）复合物的BALB/c JLS V9细胞含有6.0千碱基对大小的未整合HSV线性DNA。这些细胞还含有两种HSV闭环DNA以及MuLV编码的线性和闭环DNA。HSV 6.0千碱基对线性DNA在转染NIH 3T3小鼠成纤维细胞后诱导焦点转化，且HSV DNA的生物活性不需要辅助MuLV功能。得出了沿HSV 6.0千碱基对线性DNA的限制性内切酶切割位点的物理图谱。将该图谱与莫洛尼MuLV DNA的图谱进行比较，结果表明HSV和莫洛尼MuLV基因组在其病毒RNA 3'末端附近是相同的。
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