College of Polymer Science and Engineering, Sichuan University, Chengdu, Sichuan Province 610065, PR China.
Carbohydr Polym. 2013 Jan 30;92(1):448-54. doi: 10.1016/j.carbpol.2012.09.096. Epub 2012 Oct 6.
Biological tissues must be chemically fixed before they can be implanted in humans, due to the immediate degradation and presence of antigenicity of naturally derived tissues. To provide a crosslinking reagent which is cytocompatible and may prepare biocompatible fixed tissues, a novel crosslinking agent, alginate dialdehyde (ADA), was employed to fix biological tissues by our group. The study was to evaluate the cytocompatibility of ADA for biological tissue fixation. Glutaraldehyde and genipin counterparts were used as controls. The result suggested that the cytotoxicity of ADA was significantly lower than that of glutaraldehyde and genipin. Additionally, in the evaluation of cytotoxicity of fixed tissue itself and the residues, as well as the cell adhesion property, ADA-fixed tissue was significantly superior to its glutaraldehyde counterpart and comparable to its genipin counterpart. The results obtained in this study demonstrate that ADA is a cytocompatible crosslinking reagent for biological tissue fixation.
生物组织在植入人体之前必须经过化学固定，因为天然组织会立即降解并具有抗原性。为了提供一种细胞相容的交联试剂，用于制备生物相容性固定组织，我们小组使用了一种新型交联剂——海藻酸钠醛（ADA）。本研究旨在评估 ADA 用于生物组织固定的细胞相容性。戊二醛和京尼平作为对照。结果表明，ADA 的细胞毒性明显低于戊二醛和京尼平。此外，在固定组织本身及其残留物的细胞毒性评估以及细胞黏附特性方面，ADA 固定组织明显优于戊二醛固定组织，与京尼平固定组织相当。本研究结果表明，ADA 是一种用于生物组织固定的细胞相容交联试剂。
