肠杆菌 cloacae Z0206 产生的一种含岩藻糖的胞外多糖的结构特征。
Structure characterization of a fucose-containing exopolysaccharide produced by Enterobacter cloacae Z0206.
机构信息
College of Animal Science, Zhejiang University, Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture (East China), Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou 310058, China.
出版信息
Carbohydr Polym. 2013 Jan 30;92(1):503-9. doi: 10.1016/j.carbpol.2012.10.014. Epub 2012 Oct 12.
A novel high molecular weight (1.1 × 10(6)Da) exopolysaccharide (EPS) produced by Enterobacter cloacae Z0206 strain was isolated by column chromatography. Complete hydrolysis of the EPS followed by gas chromatography mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analyses showed that the EPS is composed of L-fucose, D-glucose, D-galactose, D-glucuronic acid and pyruvic acid in the approximate molar ratio of 2:1:3:1:1. Partial acid hydrolysis of the purified EPS followed by gel permeation chromatography (GPC) yielded a hexasaccharide. A combination of chemical analysis coupled with mass spectrometry and 1D and 2D NMR spectroscopy applied to the oligosaccharide showed that the EPS comprises a heptasaccharide repeating unit.
一株肠杆菌（Enterobacter cloacae）Z0206 菌株产生的新型高分子量（1.1×10^6Da）胞外多糖（EPS）通过柱层析法分离。EPS 的完全水解后，通过气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）分析表明，EPS 由 L-岩藻糖、D-葡萄糖、D-半乳糖、D-葡萄糖醛酸和丙酮酸以约 2:1:3:1:1 的摩尔比组成。纯化的 EPS 经部分酸水解后，通过凝胶渗透色谱（GPC）得到六糖。化学分析与质谱以及 1D 和 2D NMR 光谱的组合应用于寡糖，表明 EPS 由七糖重复单元组成。
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