通过连续传代培养蜡样芽胞杆菌和戊酮糖酸钙不动杆菌共培养物来提高 2-酮基古龙酸的产量。
Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonicigenium vulgare.
机构信息
Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.
出版信息
Bioresour Technol. 2013 Mar;132:370-3. doi: 10.1016/j.biortech.2012.10.151. Epub 2012 Nov 7.
The co-cultures of Ketogulonicigenium vulgare and Bacillus cereus were subcultured daily for a total of 150 transfers. The yield of 2-keto-gulonic acid (2-KGA) and medium pH in the co-cultures were measured. We found that the serial subcultivation increased the yield of 2-KGA from 77% (original co-culture) to 93% (the 150th transfer of transferred co-culture). The resulted strains are of industrial interests for vitamin C production.
酮古龙酸发酵球菌（Ketogulonicigenium vulgare）和蜡状芽孢杆菌（Bacillus cereus）的共培养物每天进行传代培养，共进行了 150 次传代。测量了共培养物中 2-酮古龙酸（2-KGA）的产量和培养基 pH 值。我们发现，连续传代培养将 2-KGA 的产量从 77%（原始共培养物）提高到 93%（传代共培养物的第 150 次转移）。由此产生的菌株在维生素 C 生产方面具有工业应用价值。
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