Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, PR China.
SynBio Research Platform, Collaborative Innovation Centre of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, PR China.
Sci Rep. 2016 Jun 29;6:28794. doi: 10.1038/srep28794.
Bacillus thuringiensis and Bacillus endophyticus both act as the companion bacteria, which cooperate with Ketogulonigenium vulgare in vitamin C two-step fermentation. Two Bacillus species have different morphologies, swarming motility and 2-keto-L-gulonic acid productivities when they co-culture with K. vulgare. Here, we report the complete genome sequencing of B. thuringiensis Bc601 and eight plasmids of B. endophyticus Hbe603, and carry out the comparative genomics analysis. Consequently, B. thuringiensis Bc601, with greater ability of response to the external environment, has been found more two-component system, sporulation coat and peptidoglycan biosynthesis related proteins than B. endophyticus Hbe603, and B. endophyticus Hbe603, with greater ability of nutrients biosynthesis, has been found more alpha-galactosidase, propanoate, glutathione and inositol phosphate metabolism, and amino acid degradation related proteins than B. thuringiensis Bc601. Different ability of swarming motility, response to the external environment and nutrients biosynthesis may reflect different companion mechanisms of two Bacillus species. Comparative genomic analysis of B. endophyticus and B. thuringiensis enables us to further understand the cooperative mechanism with K. vulgare, and facilitate the optimization of bacterial consortium.
苏云金芽孢杆菌和内生芽孢杆菌均为伴生细菌，与酮古龙酸杆菌合作进行维生素 C 两步发酵。当这两种芽孢杆菌与酮古龙酸杆菌共培养时，它们具有不同的形态、泳动能力和 2-酮-L-古龙酸的产量。在此，我们报道了苏云金芽孢杆菌 Bc601 的全基因组测序结果和内生芽孢杆菌 Hbe603 的 8 个质粒，并进行了比较基因组学分析。结果表明，苏云金芽孢杆菌 Bc601 具有更强的对外界环境的响应能力，其拥有更多的双组分系统、孢子衣和肽聚糖生物合成相关蛋白，而内生芽孢杆菌 Hbe603 具有更强的营养物质生物合成能力，其拥有更多的α-半乳糖苷酶、丙酸盐、谷胱甘肽和肌醇磷酸盐代谢以及氨基酸降解相关蛋白。不同的泳动能力、对外界环境的响应能力和营养物质生物合成能力可能反映了两种芽孢杆菌不同的伴生机制。内生芽孢杆菌和苏云金芽孢杆菌的比较基因组分析使我们能够进一步了解与酮古龙酸杆菌的合作机制，并有助于优化细菌联合体。
Zotero 插件
