Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy.
Lung Cancer. 2013 Feb;79(2):180-6. doi: 10.1016/j.lungcan.2012.11.004. Epub 2012 Dec 4.
Survivin is expressed in lung cancer and in most cancer tissues and has a significant impact on prognosis. This work aimed to comparatively assess survivin expression and significance in Non-Small (NSCLC) and Small Cell Lung Cancers (SCLC). Sixty-five NSCLC and 35 SCLC samples were analyzed by semi-quantitative real-time RT-PCR. Survivin mRNA levels were significantly higher in tumors than in normal tissue, and in SCLC than in NSCLC samples. Immunohistochemistry and FISH analyses were performed in 59 and 26 tumor specimens, respectively. In SCLC survivin was only present in cytoplasm, while in some NSCLC cases it also showed nuclear or mixed patterns. FISH analysis did not disclose survivin gene amplification, except for one NSCLC case. Finally, 90 samples were genotyped for the -31G/C SNP of survivin promoter by direct sequencing; the -31G/C SNP genotype status showed a significant association only with nodal NSCLC metastasis, but not with survivin expression in any tumor group. A better prognosis was correlated to higher levels of survivin mRNA and to the presence of at least one G allele at -31 SNP in NSCLC, while these parameters did not correlate with overall survival in SCLC. Moreover, this SNP would appear to have no effect on the risk of lung cancer in our samples. The different prognostic role played by survivin in NSCLC and SCLC highlights the biological differences between these lung tumor histotypes and stresses the need to clarify the molecular pathways leading to their neoplastic transformation.
Survivin 在肺癌和大多数癌症组织中表达，对预后有重要影响。本工作旨在比较评估非小细胞肺癌（NSCLC）和小细胞肺癌（SCLC）中 survivin 的表达和意义。通过半定量实时 RT-PCR 分析了 65 例 NSCLC 和 35 例 SCLC 样本。Survivin mRNA 水平在肿瘤组织中明显高于正常组织，在 SCLC 中高于 NSCLC 样本。分别对 59 例和 26 例肿瘤标本进行了免疫组化和 FISH 分析。在 SCLC 中，survivin 仅存在于细胞质中，而在一些 NSCLC 病例中也存在核或混合模式。FISH 分析未发现 survivin 基因扩增，除了一个 NSCLC 病例。最后，通过直接测序对 90 例样本的 survivin 启动子 -31G/C SNP 进行了基因分型；-31G/C SNP 基因型状态与淋巴结 NSCLC 转移显著相关，但与任何肿瘤组中的 survivin 表达无关。较高的 survivin mRNA 水平与 NSCLC 中至少存在一个 G 等位基因与更好的预后相关，而这些参数与 SCLC 的总生存期无关。此外，该 SNP 似乎对我们样本中的肺癌风险没有影响。Survivin 在 NSCLC 和 SCLC 中发挥的不同预后作用突出了这些肺肿瘤组织类型之间的生物学差异，并强调需要阐明导致它们发生肿瘤转化的分子途径。
