Laboratory of Separation and Reaction Engineering, Dpt. de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, s/n 4200-465 Porto, Portugal.
J Chromatogr A. 2013 Jan 4;1271(1):10-6. doi: 10.1016/j.chroma.2012.11.028. Epub 2012 Nov 19.
Partition ratios of several ionic compounds in 20 different polymer/polymer aqueous two-phase systems (ATPS) containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, were determined. The differences between the electrostatic properties of the phases in all the ATPS were estimated from partitioning of the homologous series of dinitrophenylated-amino acids. Also the solvatochromic solvent parameters characterizing the solvent dipolarity/polarizability (π*), solvent hydrogen-bond donor acidity (α), and solvent hydrogen-bond acceptor basicity (β) of aqueous media were measured in the coexisting phases of the ATPS. The solute-specific coefficients for the compounds examined were determined by the multiple linear regression analysis using the modified linear solvation energy relationship equation. The minimal number of ATPS necessary for determination of the coefficients was established and 10 ATPS were selected as a reference ATPS set. The solute-specific coefficients values obtained with this reference set of ATPS were used to predict the partition ratios for the compounds in 10 ATPS not included in the reference set. The predicted partition ratios values were compared to those determined experimentally and found to be in good agreement. It is concluded that the presented model of solute-solvent interactions as the driving force for solute partitioning in polymer/polymer ATPS describes experimental observations with 90-95% accuracy.
在含有 0.15 M NaCl 的 0.01 M 磷酸盐缓冲液，pH 值为 7.4 的 20 种不同聚合物/聚合物双水相体系 (ATPS) 中，测定了几种离子化合物的分配比。从同系二硝基苯氨基酸的分配估计了所有 ATPS 中相的静电特性差异。还测量了在 ATPS 的共存相中表征溶剂偶极/极化度（π*）、溶剂氢键供体酸度（α）和溶剂氢键接受碱性（β）的溶剂变色溶剂参数。使用改进的线性溶剂化能量关系方程，通过多元线性回归分析确定了所研究化合物的特定溶质系数。确定系数所需的最小 ATPS 数量已建立，并且选择了 10 种 ATPS 作为参考 ATPS 集。使用该参考 ATPS 集获得的特定溶质系数值用于预测不在参考集中的 10 种 ATPS 中化合物的分配比。将预测的分配比值与实验确定的值进行比较，发现它们非常吻合。得出的结论是，作为聚合物/聚合物双水相体系中溶质分配驱动力的溶质-溶剂相互作用模型以 90-95%的准确度描述了实验观察结果。
