Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Int J Biol Macromol. 2013 Mar;54:90-8. doi: 10.1016/j.ijbiomac.2012.11.031. Epub 2012 Dec 5.
Green tea polyphenols (GTPs) are found to be potent inhibitors of amyloid fibril formation. We report the effective inhibitory property of (-)-epicatechin gallate (ECG) during the alkali-salt induced fibrillogenesis of hen egg white lysozyme (HEWL) at 37 °C. Spectroscopic techniques such as fluorescence, circular dichroism and microscopic images show that (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) show moderate inhibition of fibrillation with ECG as the most potent polyphenol. Aromatic interactions, hydrophobic interactions, the radical scavenging activity and autoxidation of polyphenols are likely to be the major reasons for ECG being the most effective inhibitor.
绿茶多酚（GTPs）被发现是抑制淀粉样纤维形成的有效物质。我们报道了在 37°C 下，(-)-表没食子儿茶素没食子酸酯（ECG）对碱性盐诱导的鸡卵清溶菌酶（HEWL）纤维原纤维形成的有效抑制作用。荧光、圆二色性和显微镜图像等光谱技术表明，(-)-表儿茶素（EGC）、(-)-表没食子儿茶素没食子酸酯（ECG）和(-)-表没食子儿茶素没食子酸酯（EGCG）对纤维原纤维形成有中等抑制作用，其中 ECG 是最有效的多酚。芳香相互作用、疏水相互作用、多酚的清除自由基活性和自动氧化可能是 ECG 成为最有效抑制剂的主要原因。
