University of Melbourne, Department of Paediatrics and Murdoch Childrens Research Institute, Parkville, Victoria 3052, Australia.
Osteoarthritis Cartilage. 2013 Feb;21(2):401-4. doi: 10.1016/j.joca.2012.11.013. Epub 2012 Dec 4.
Recent analyses of Col2a1-Cre; ROSA26R reporter mice showed that synovial fibroblasts in 7-day mice were LacZ positive, due to a history of Col2a1-Cre expression conferred by their origin in the interzone of the developing joint. We have examined LacZ staining in adult Col2a1-Cre(+/0); ROSA26R(LacZ) mice, with and without inflammatory arthritis, and found that synovial fibroblasts in normal and inflamed synovium are LacZ positive, but Cre negative. Our results suggest that Cre-mediated recombination in joint interzone cells during development endure in adult synovial cells despite the absence of ongoing Cre expression. These findings have important implications and applications for the study of synovial inflammation in models of experimental arthritis.
最近对 Col2a1-Cre;ROSA26R 报告小鼠的分析表明，在 7 天龄的小鼠中，由于其起源于发育关节的间充质区，具有 Col2a1-Cre 表达的历史，滑膜成纤维细胞呈 LacZ 阳性。我们检查了 Col2a1-Cre(+/0);ROSA26R(LacZ)成年小鼠的 LacZ 染色，有和没有炎症性关节炎，并发现正常和炎症性滑膜中的滑膜成纤维细胞呈 LacZ 阳性，但 Cre 阴性。我们的结果表明，尽管不存在持续的 Cre 表达，但在发育过程中关节间充质区细胞中的 Cre 介导的重组在成年滑膜细胞中持续存在。这些发现对实验性关节炎模型中滑膜炎症的研究具有重要意义和应用价值。
