Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University, Busan 609-735, Republic of Korea.
Food Chem Toxicol. 2013 Mar;53:105-11. doi: 10.1016/j.fct.2012.11.002. Epub 2012 Nov 28.
Protective effects of Tartary buckwheat (TB) and common buckwheat (CB) on amyloid beta (Aβ)-induced impairment of cognition and memory function were investigated in vivo in order to identify potential therapeutic agents against Alzheimer's disease (AD) and its associated progressive memory deficits, cognitive impairment, and personality changes. An in vivo mouse model of AD was created by injecting the brains of ICR mice with Aβ(25-35), a fragment of the full-length Aβ protein. Damage of mice recognition ability through following Aβ(25-35) brain injections was confirmed using the T-maze test, the object recognition test, and the Morris water maze test. Results of behavior tests in AD model showed that oral administration of the methanol (MeOH) extracts of TB and CB improved cognition and memory function following Aβ(25-35) injections. Furthermore, in groups receiving the MeOH extracts of TB and CB, lipid peroxidation was significantly inhibited, and nitric oxide levels in tissue, which are elevated by injection of Aβ(25-35), were also decrease. In particular, the MeOH extract of TB exerted a stronger protective activity than CB against Aβ(25-35)-induced memory and cognition impairment. The results indicate that TB may play a promising role in preventing or reversing memory and cognition loss associated with Aβ(25-35)-induced AD.
为了寻找治疗阿尔茨海默病（AD）及其相关的进行性记忆缺陷、认知障碍和人格变化的潜在治疗药物，本研究从体内水平研究了苦荞（TB）和甜荞（CB）对淀粉样β（Aβ）诱导的认知和记忆功能损伤的保护作用。通过向 ICR 小鼠脑内注射 Aβ（25-35），即全长 Aβ 蛋白的片段，构建 AD 体内小鼠模型。通过 T 迷宫测试、物体识别测试和 Morris 水迷宫测试，确认 Aβ（25-35）脑内注射对小鼠识别能力的损害。AD 模型的行为测试结果表明，TB 和 CB 的甲醇（MeOH）提取物经口服给药可改善 Aβ（25-35）注射后的认知和记忆功能。此外，在接受 TB 和 CB 的 MeOH 提取物的组中，脂质过氧化明显受到抑制，并且由 Aβ（25-35）注射引起的组织中一氧化氮水平也降低。特别是，TB 的 MeOH 提取物对 Aβ（25-35）诱导的记忆和认知损伤的保护作用强于 CB。结果表明，TB 可能在预防或逆转与 Aβ（25-35）诱导的 AD 相关的记忆和认知丧失方面发挥有前景的作用。
