State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Antiviral Res. 2013 Feb;97(2):145-53. doi: 10.1016/j.antiviral.2012.11.009. Epub 2012 Dec 7.
We designed a series of epitope proteins containing the G-H loops of three topotypes of foot-and-mouth disease virus (FMDV) serotype O and promiscuous artificial Th sites and selected one epitope protein (designated as B4) with optimal immunogenicity and cross-reactivity. Three out of five pigs immunized intramuscularly with this B4 were protected against virulent FMDV challenge after a single inoculation, while all pigs co-immunized with B4 and polyinosinic-cytidylic acid [poly(I:C)] conferred complete protection following FMDV challenge. Additionally, we demonstrated that all pigs co-immunized with B4 and poly(I:C) elicited FMDV-specific neutralizing antibodies, total IgG antibodies, type I interferon (IFN-α/β) and cytokines IFN-γ. In contrast, some pigs immunized with B4 alone produced parameters mentioned above, while some not, suggesting that poly(I:C) reduced animal-to-animal variations in both cellular and humoral responses often observed in association with epitope-based vaccines and up-regulated T-cell immunity often poorly observed in protein-based vaccines. We propose that poly(I:C) is an effective adjuvant for this epitope-based vaccine of FMDV. This combination could yield an effective and safe candidate vaccine for the control and eradication of FMD in pigs.
我们设计了一系列包含三种口蹄疫病毒（FMDV）血清型 O 拓扑结构 G-H 环和混杂人工 Th 位点的表位蛋白，并选择了一种具有最佳免疫原性和交叉反应性的表位蛋白（命名为 B4）。5 头肌肉注射 B4 的猪中有 3 头在单次接种后可免受强毒 FMDV 的攻击，而所有 B4 和聚肌胞苷酸[poly(I:C)]共同免疫的猪在 FMDV 攻击后均可完全保护。此外，我们证明，所有 B4 和 poly(I:C)共同免疫的猪均能产生 FMDV 特异性中和抗体、总 IgG 抗体、I 型干扰素（IFN-α/β）和细胞因子 IFN-γ。相比之下，一些单独接种 B4 的猪产生了上述参数，而一些则没有，这表明 poly(I:C)减少了基于表位的疫苗常观察到的细胞和体液反应的个体间差异，并上调了通常在基于蛋白的疫苗中观察到的 T 细胞免疫。我们提出 poly(I:C)是这种基于表位的 FMDV 疫苗的有效佐剂。这种组合可能会产生一种有效和安全的候选疫苗，用于控制和根除猪 FMD。
