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本文引用的文献

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Genetic selection designed to stabilize proteins uncovers a chaperone called Spy.遗传选择设计用于稳定蛋白质，揭示了一种称为 Spy 的伴侣蛋白。
Nat Struct Mol Biol. 2011 Mar;18(3):262-9. doi: 10.1038/nsmb.2016. Epub 2011 Feb 13.
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Molecular basis for polyol-induced protein stability revealed by molecular dynamics simulations.多元醇诱导蛋白质稳定性的分子基础：分子动力学模拟研究
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Chaperone networks: tipping the balance in protein folding diseases.伴侣蛋白网络：在蛋白质折叠疾病中改变平衡。
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Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes.半胱氨酸-β-合酶（CBS）突变体的化学伴侣救援：八种 CBS 突变酶的纯化和表征。
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Urea denatured state ensembles contain extensive secondary structure that is increased in hydrophobic proteins.尿素变性状态集合包含广泛的二级结构，在疏水蛋白中增加。
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Hydrogen bonding progressively strengthens upon transfer of the protein urea-denatured state to water and protecting osmolytes.氢键在蛋白质从尿素变性状态转移到水中并被保护渗透物保护时逐渐增强。
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Mechanisms of protein stabilization and prevention of protein aggregation by glycerol.甘油对蛋白质的稳定作用及防止蛋白质聚集的机制。
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Chemical chaperone and inhibitor discovery: potential treatments for protein conformational diseases.化学伴侣和抑制剂的发现：蛋白质构象疾病的潜在治疗方法。
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在体检测和定量分析增强蛋白质稳定性的化学物质。

In vivo detection and quantification of chemicals that enhance protein stability.

机构信息

Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.

出版信息

Anal Biochem. 2013 Mar 1;434(1):181-6. doi: 10.1016/j.ab.2012.11.022. Epub 2012 Dec 5.

DOI:10.1016/j.ab.2012.11.022
PMID:23219982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3670414/
Abstract

We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.

摘要

我们设计了一种蛋白质折叠传感器，将蛋白质稳定性与 TEM-1 β-内酰胺酶活性联系起来。在含有这些传感器的细菌生长培养基中添加渗透剂和其他具有化学伴侣活性的化合物，可使β-内酰胺酶活性在剂量依赖性的方式下增加 207 倍。这使得能够快速检测和灵敏地定量增强体内蛋白质稳定性的化合物。