Department of Zoology, Banaras Hindu University, Varanasi-221005, India.
Exp Gerontol. 2013 Feb;48(2):168-82. doi: 10.1016/j.exger.2012.11.012. Epub 2012 Dec 3.
Reactive oxygen species (ROS) have been proposed to play an important role in balancing the pro- and antioxidant homeostasis during aging. Melatonin has been suggested as an effective free radical scavenger that might have a role during the process of aging. We observed, that melatonin administration (25 μg/100 g body weight for 30 days) significantly augments the activity of anti-oxidative enzymes like superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in the plasma, spleen and bone marrow (BM) of young (6 weeks), adult (30 weeks) and old aged (2.5 years) male golden hamster, Mesocricetus auratus. A sharp decline in generation of ROS was observed in peripheral blood mononuclear cells (PBMC) and splenocytes upon melatonin administration in different age group of hamsters. Reduction in the level of thiobarbituric acid-reactive substances (TBARS) and total nitrite and nitrate concentration as metabolites and indicators of nitric oxide (NO) in plasma, spleen and BM were observed along with night time (22:00 h) melatonin concentration in different age group of hamsters after administration of melatonin and compared to the control group (treated with 0.9% saline). General immune parameters like proliferation of splenocytes, PBMC and colony forming ability of GM-CFU were observed following melatonin treatment in different age group, although it was low only in aged hamsters compared to the young and adult. Our data indicates that the age related increase of oxidative load and simultaneously augments the general immunity in aged hamsters.
活性氧 (ROS) 被认为在衰老过程中在促进和抗氧化平衡中发挥重要作用。褪黑素已被提议作为一种有效的自由基清除剂，可能在衰老过程中发挥作用。我们观察到，褪黑素给药（25μg/100g 体重，30 天）可显著增强年轻（6 周）、成年（30 周）和老年（2.5 岁）雄性金黄仓鼠 Mesocricetus auratus 血浆、脾脏和骨髓（BM）中抗氧化酶如超氧化物歧化酶 (SOD)、过氧化氢酶和谷胱甘肽过氧化物酶 (GPx) 的活性。在不同年龄组的仓鼠中给予褪黑素后，观察到外周血单核细胞 (PBMC) 和脾细胞中 ROS 的产生明显下降。在不同年龄组的仓鼠中给予褪黑素后，血浆、脾脏和 BM 中丙二醛 (TBARS) 和总亚硝酸盐和硝酸盐浓度作为代谢物和一氧化氮 (NO) 的指标以及夜间（22:00h）褪黑素浓度降低。与对照组（用 0.9%生理盐水处理）相比，在不同年龄组的仓鼠中观察到一般免疫参数如脾细胞、PBMC 的增殖和 GM-CFU 的集落形成能力，尽管与年轻和成年相比，老年仓鼠中的这种作用较低。我们的数据表明，衰老相关的氧化负荷增加，同时增强了老年仓鼠的一般免疫力。
