Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Medical Education Program for Human Resources, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
Biochem Biophys Res Commun. 2013 Jan 11;430(2):554-9. doi: 10.1016/j.bbrc.2012.11.114. Epub 2012 Dec 7.
Laminar shear stress (LSS) due to blood flow contributes to the maintenance of endothelial health by multiple mechanisms including promotion of wound healing. The present study examined the hypothesis that the induction of water channel aquaporin 1 (AQP1) expression by LSS might be functionally associated with endothelial wound healing. When human umbilical vein endothelial cells were exposed to LSS at 12 dyn cm(-2) for 24h, significant increases in AQP1 expression were observed at the mRNA and protein levels as compared with static control. In the in vitro scratch wound healing assay, LSS treatments before and after wound creation enhanced endothelial wound healing and this effect was significantly attenuated by selective suppression of AQP1 expression using small interfering RNA. Ectopic expression of AQP1 enhanced wound healing in the absence of LSS. This study demonstrated that LSS stimulates the endothelial expression of AQP1 that plays a role in wound healing.
层流切应力（LSS）通过多种机制促进伤口愈合，有助于维持内皮细胞健康。本研究通过实验来验证假设，即 LSS 诱导水通道蛋白 aquaporin 1（AQP1）的表达可能与内皮细胞伤口愈合功能相关。当人脐静脉内皮细胞在 12 dyn cm(-2)的层流切应力下孵育 24 小时后，与静态对照组相比，AQP1 的 mRNA 和蛋白表达水平显著增加。在体外划痕实验中，在创伤形成前后进行 LSS 处理可增强内皮细胞的伤口愈合能力，而使用小干扰 RNA 选择性抑制 AQP1 的表达可显著减弱这一作用。AQP1 的异位表达可在无 LSS 的情况下增强伤口愈合。本研究表明，LSS 刺激内皮细胞表达 AQP1，在伤口愈合中发挥作用。
