Klaus S, Casteilla L, Bouillaud F, Raimbault S, Ricquier D
Centre National de la Recherche Scientifique, Centre de Recherches sur la Nutrition, Meudon-Bellevue, France.
Biochem Biophys Res Commun. 1990 Mar 16;167(2):784-9. doi: 10.1016/0006-291x(90)92094-g.
Non shivering thermogenesis of brown adipose tissue is due to the uncoupling protein (UCP), located in the inner mitochondrial membrane, which functions as a proton translocator and can thus uncouple mitochondrial respiration. We describe here the expression of UCP in Xenopus laevis oocytes after injection of UCP mRNA, which was transcribed in vitro. UCP seems to be correctly transported into mitochondria and integrated into the membrane, but we were not able to establish definitely the functionality of this UCP. We conclude that this expression system could be suitable for the study of the mitochondrial import mechanism but not for the examination of physiological properties of UCP.
棕色脂肪组织的非颤抖性产热是由于位于线粒体内膜的解偶联蛋白（UCP），它作为质子转运体发挥作用，从而使线粒体呼吸解偶联。我们在此描述了体外转录的UCP mRNA注射到非洲爪蟾卵母细胞后UCP的表达情况。UCP似乎能正确转运至线粒体并整合到膜中，但我们无法确切确定这种UCP的功能。我们得出结论，该表达系统可能适用于研究线粒体导入机制，但不适用于检测UCP的生理特性。
