Nasu M, Yutsudo M, Toyoshima K
Microbiol Immunol. 1979;23(8):779-90. doi: 10.1111/j.1348-0421.1979.tb00519.x.
The size of genomic RNA in PR-RSV A passaged in chick embryo fibroblasts (CEF) or quail embryo fibroblasts (QEF) was determined by gel electrophoresis. The results showed that 3 undiluted passages resulted in heterogeneity of RNA. The heterogeneity of the smaller incomplete RNAs in the virus stock was decreased by diluted passage or cloning, but RNA of the b subunit size and a subunit RNA of complete genome size were relatively stable. These heterogeneous RNAs were characterized by hybridization analysis. The RNAs from 4 peaks hybridized with both cDNAtotal and cDNAsrc to appreciable extents, indicating that they were derived from viral RNA and that at least some of them contained the src sequence. This finding and the failure to isolate a td mutant from the undiluted-passaged virus stock or from some subclones that had a and b subunits of RNA indicate that the td virus was only a minor constituent of the incomplete virus population caused by undiluted passages. Some viruses with incomplete RNA in the virus stock could produce foci with the aid of td B77 or RAV-60. The emergence of rd viruses by undiluted passages was indicated.
通过凝胶电泳测定了在鸡胚成纤维细胞（CEF）或鹌鹑胚成纤维细胞（QEF）中传代的PR-RSV A的基因组RNA大小。结果表明，3次未稀释传代导致RNA出现异质性。病毒原液中较小的不完全RNA的异质性通过稀释传代或克隆而降低，但b亚基大小的RNA和完整基因组大小的a亚基RNA相对稳定。通过杂交分析对这些异质性RNA进行了表征。来自4个峰的RNA与总cDNA和src cDNA均有明显程度的杂交，表明它们源自病毒RNA，并且其中至少一些包含src序列。这一发现以及未能从未稀释传代的病毒原液或具有RNA a和b亚基的一些亚克隆中分离出td突变体，表明td病毒只是未稀释传代导致的不完全病毒群体中的次要成分。病毒原液中一些具有不完全RNA的病毒可借助td B77或RAV-60产生蚀斑。表明未稀释传代出现了rd病毒。
