Castelli C, Mazzucchelli F, Sensi M, Carbone G, Parmiani G
Division of Experimental Oncology D, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Exp Clin Immunogenet. 1990;7(2):129-35.
The objective of our study was to determine whether and how frequently MHC class I gene alterations occur in fibrosarcomas induced in BALB/c mice by the carcinogen 3-methylcholanthrene (3-MCA). Southern blot was performed to analyze the genomic organization of class I genes in a panel of twenty tumors of different immunogenic strength used at early in vivo passages. Our results rule out the presence of gross rearrangements or deletions in the H-2 class I genes of the tumors tested and indicate that the frequency of detectable class I alterations giving rise to restriction fragments length polymorphism (RFLP) should be very low.
我们研究的目的是确定在致癌物3-甲基胆蒽（3-MCA）诱导的BALB/c小鼠纤维肉瘤中，MHC I类基因改变是否发生以及发生的频率如何。对一组体内早期传代的、具有不同免疫原性强度的20个肿瘤进行Southern印迹分析，以研究I类基因的基因组结构。我们的结果排除了受试肿瘤的H-2 I类基因存在大规模重排或缺失的可能性，并表明导致限制性片段长度多态性（RFLP）的可检测I类改变的频率应该非常低。
