Graduate School of Medicine, Kyoto University, Yoshida-konoe, Sakyo-ku, Kyoto, Japan.
Adv Exp Med Biol. 2013;961:193-201. doi: 10.1007/978-1-4614-4756-6_16.
Mitochondria Na(+)-Ca(2+) exchange (NCX(mit)) was first discovered by Carafoli et al. in 1974. Thereafter, the mechanisms and roles of NCX(mit) have been extensively studied. We review NCX(mit) in cardiomyocytes and lymphocytes by presenting our recent studies on it. Studies of NCX(mit) in rat ventricular cells demonstrated that NCX(mit) is voltage dependent and electrogenic. A targeted knockdown and knockout of NCLX in HL-1 cardiomyocytes and B lymphocytes, respectively, significantly reduced the NCX(mit) activity, indicating that NCLX is a major component of NCX(mit) in these cells. The store-operated Ca(2+) entry was greatly attenuated in NCLX knockout lymphocytes, suggesting that substantial amount of Ca(2+) enters into mitochondria and is released to cytosol via NCX(mit). NCX(mit) or NCLX has pivotal roles in Ca(2+) handling in mitochondria and cytoplasm.
线粒体钠钙交换（NCX(mit)）于 1974 年由 Carafoli 等人首次发现。此后，NCX(mit)的机制和作用得到了广泛的研究。我们通过介绍最近对心肌细胞和淋巴细胞中 NCX(mit)的研究，对其进行了综述。对大鼠心室细胞中 NCX(mit)的研究表明，NCX(mit)是电压依赖性和电致性的。在 HL-1 心肌细胞和 B 淋巴细胞中分别靶向敲低和敲除 NCLX，显著降低了 NCX(mit)的活性，表明 NCLX 是这些细胞中 NCX(mit)的主要组成部分。NCLX 敲除淋巴细胞中的储存操纵性 Ca(2+)内流大大减弱，表明大量 Ca(2+)进入线粒体，并通过 NCX(mit)释放到细胞质中。NCX(mit)或 NCLX 在线粒体和细胞质中的 Ca(2+)处理中起着关键作用。
