硫氰酸根离子对用于评估α-淀粉酶活性的淀粉-碘反应的影响。
Influence of thiocyanate ions on starch-iodine reaction used for the estimation of alpha-amylase activity.
作者信息
Lathia D, Brendebach M
出版信息
Clin Chim Acta. 1978 Jan 16;82(3):209-14. doi: 10.1016/0009-8981(78)90001-3.
PMID:23226
Abstract
A strong simulating effect of thiocyanate ions during the estimation of alpha-amylase by the amyloclastic method is shown. Thiocyanate ions themselves catalyse the decolorization of the starch-iodine complex. This may lead to wrong values during determination of alpha-amylase by this method. The rate of thiocyanate-iodine reaction depends upon the hydrogen ion concentration of the iodine reagent.
摘要
结果表明，在采用淀粉水解法测定α-淀粉酶时，硫氰酸根离子具有强烈的模拟效应。硫氰酸根离子本身会催化淀粉-碘复合物的褪色反应。这可能导致用该方法测定α-淀粉酶时得出错误的值。硫氰酸根离子与碘的反应速率取决于碘试剂的氢离子浓度。
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