Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
Transfusion. 2013 Aug;53(8):1798-807. doi: 10.1111/trf.12010. Epub 2012 Dec 11.
The Reveos system (Terumo BCT) is a fully automated device able to process four whole blood (WB) units simultaneously into a plasma unit, a red blood cell (RBC) unit, and an interim platelet (PLT) unit (IPU). Multiple IPUs can be pooled to form a transfusable PLT product. The aim of our study was to evaluate the quality of components made with the Reveos system from either fresh (2-8 hr) or overnight-held WB.
A prototype of the Reveos system was used to process WB. RBCs were resuspended in SAGM, leukoreduced, and assayed for in vitro quality variables during a 42-day storage period at 2 to 6 °C. Twenty-four-hour in vivo recovery was determined on Day 42. Plasma was assayed for cellular contamination and activation variables. IPUs were pooled with SSP+ additive solution for in vitro quality assessments during a 7-day storage period at room temperature.
Reveos-produced RBCs and plasma units met the predefined requirements. RBC recovery was superior to control units. On Day 42, hemolysis was below 0.8% and in vivo recovery was above 75% for all RBCs. Cellular contamination was lower for Reveos-produced plasma. PLT yield was higher with overnight-stored WB. PLT quality was well maintained during storage with no significant differences between the two groups.
Blood components prepared with the Reveos from fresh or overnight-held WB meet quality criteria without any relevant difference between the two groups. The Reveos system has the potential to increase efficacy and standardization of blood component preparation.
Reveos 系统（泰尔茂 BCT）是一种全自动设备，能够同时处理四个全血（WB）单位，将其制成血浆单位、红细胞（RBC）单位和临时血小板（PLT）单位（IPU）。多个 IPU 可以汇集形成可输血小板产品。我们的研究目的是评估使用 Reveos 系统从新鲜（2-8 小时）或隔夜保存的 WB 制备的成分的质量。
使用 Reveos 系统的原型来处理 WB。RBC 悬浮于 SAGM 中，经白细胞减少处理，并在 2 至 6°C 的 42 天储存期内检测体外质量变量。第 42 天测定 24 小时体内恢复情况。检测血浆中的细胞污染和激活变量。将 IPU 与 SSP+添加剂溶液混合，在室温下进行 7 天的体外质量评估。
Reveos 生产的 RBC 和血浆单位符合预定要求。RBC 恢复率优于对照单位。第 42 天，所有 RBC 的溶血率均低于 0.8%，体内恢复率均高于 75%。Reveos 生产的血浆中细胞污染较低。使用隔夜保存的 WB 可提高 PLT 产量。PLT 质量在储存期间得到很好的维持，两组之间没有显著差异。
使用 Reveos 从新鲜或隔夜保存的 WB 制备的血液成分符合质量标准，两组之间没有任何显著差异。Reveos 系统有可能提高血液成分制备的效率和标准化程度。
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