Thomas Stephen, Beard Martin, Garwood Margaret, Callaert Martine, Waeg Geert van, Cardigan Rebecca
Components Development Laboratory, NHS Blood & Transplant, Brentwood, UK.
Transfusion. 2008 Dec;48(12):2515-24. doi: 10.1111/j.1537-2995.2008.01911.x. Epub 2008 Sep 4.
The Atreus 2C+ system (Gambro BCT) automates whole blood (WB) processing into a single device. This study compared the quality of red blood cells (RBCs), fresh-frozen plasma (FFP), and buffy coats (BCs) made from WB held with or without active cooling.
WB was collected into Atreus disposables and stored with (n = 20) or without (n = 20) active cooling for 14 to 18 hours at 22 +/- 2 degrees C before processing with the Atreus. Two RBC leukodepletion filters were assessed, and markers of RBC quality were tested to Day 42. BCs were held for 3 hours before testing, plasma was tested, and samples were frozen for coagulation analysis.
RBCs met UK specifications for volume, hemoglobin content (48 +/- 5 g), and hematocrit (Hct). Hemolysis, adenosine triphosphate, 2,3-diphosphoglycerate, potassium, glucose, and lactate throughout storage were all within expected ranges. No differences were seen in RBC produced from WB held with or without active cooling. FFP units met UK specification for volume, total protein, cellular contamination, and coagulation factors. No differences were seen in FFP produced from WB held with or without active cooling. The Hct of BCs produced from WB held without active cooling was lower than in BCs from WB held with active cooling; no differences in activation were seen.
From these in vitro data, blood components produced using the Atreus appear suitable for clinical use, with no clinically significant difference in the quality of components from WB held at ambient temperature overnight with or without active cooling.
Atreus 2C+系统（甘布罗BCT公司）可将全血处理自动化集成于单一设备中。本研究比较了在有或无主动冷却条件下保存的全血所制备的红细胞、新鲜冰冻血浆和 Buffy 层的质量。
将全血收集至Atreus一次性用品中，在22±2℃下，有（n = 20）或无（n = 20）主动冷却的情况下保存14至18小时，然后用Atreus进行处理。评估了两种红细胞白细胞去除过滤器，并对红细胞质量标志物进行检测至第42天。Buffy层在检测前保存3小时，对血浆进行检测，并将样本冷冻进行凝血分析。
红细胞的体积、血红蛋白含量（48±5g）和血细胞比容符合英国标准。整个储存过程中的溶血、三磷酸腺苷、2,3 - 二磷酸甘油酸、钾、葡萄糖和乳酸均在预期范围内。有或无主动冷却保存的全血所产生的红细胞之间未见差异。新鲜冰冻血浆单位的体积、总蛋白、细胞污染和凝血因子符合英国标准。有或无主动冷却保存的全血所产生的新鲜冰冻血浆之间未见差异。无主动冷却保存的全血所产生的Buffy层的血细胞比容低于有主动冷却保存的全血所产生的Buffy层；激活方面未见差异。
根据这些体外数据，使用Atreus生产的血液成分似乎适合临床使用，在有或无主动冷却的情况下，在室温下过夜保存的全血所产生的成分质量在临床上无显著差异。
