dGuo-C8-AAF、dGuo-C8-AF以及dGuo-C8-AF的咪唑环开环产物的荧光发射特性表明，这些不同化合物具有不同的动态结构。

The fluorescence emission properties of dGuo-C8-AAF, dGuo-C8-AF and the imidazole ring-opened products of dGuo-C8-AF indicate a different dynamic structure for the various compounds.

作者信息

van Houte L P, Retèl J, Westra J G, van Grondelle R

机构信息

Free University, Department of Biophysics, The Netherlands.

出版信息

Carcinogenesis. 1990 Apr;11(4):701-4. doi: 10.1093/carcin/11.4.701.

DOI:10.1093/carcin/11.4.701

PMID:2323010

Abstract

The reactive metabolites of the carcinogenic N-acetyl-2-aminofluorene (AAF) form adducts with the guanine base of DNA. The fluorescence emission characteristics of N-(deoxy-guanosin-8-yl)-N-acetyl-2-aminofluorene (dGuo-C8-AAF), N-(deoxyguanosin-8-yl)-2-aminofluorene (dGuo-C8-AF) and the two N7 = C8 imidazole ring-opened products of dGuo-C8-AF (ro-dGuo-C8-AF I + II) were investigated and related to their conformational properties. The dGuo-C8-AF adduct (phi F congruent to 4-5 X 10(-4) shows a broad and structureless emission band, which is attributed to the formation of an excited-state complex. In contrast, the emission spectra of dGuo-C8-AAF (phi F congruent to 1.10(-4] and both ro-dGuo-C8-AF compounds [phi F(ro-dGuo-C8-AF I) congruent to 4.10(-3); phi F(ro-dGuo-C8-AF II) congruent to 4.10(-4)] are narrow. This indicates that dGuo-C8-AAF and ro-dGuo-C8-AF I + II do not decay into an exciplex as occurs in dGuo-C8-AF. The spectroscopic features are discussed in terms of the differences in the dynamic structure of the various compounds.

摘要

致癌物质N-乙酰-2-氨基芴（AAF）的反应性代谢产物与DNA的鸟嘌呤碱基形成加合物。研究了N-（脱氧鸟苷-8-基）-N-乙酰-2-氨基芴（dGuo-C8-AAF）、N-（脱氧鸟苷-8-基）-2-氨基芴（dGuo-C8-AF）以及dGuo-C8-AF的两种N7 = C8咪唑环开环产物（ro-dGuo-C8-AF I + II）的荧光发射特性，并将其与它们的构象性质相关联。dGuo-C8-AF加合物（φF约为4 - 5×10⁻⁴）显示出宽且无结构的发射带，这归因于激发态复合物的形成。相比之下，dGuo-C8-AAF（φF约为1.10⁻⁴）以及两种ro-dGuo-C8-AF化合物[φF（ro-dGuo-C8-AF I）约为4.10⁻³；φF（ro-dGuo-C8-AF II）约为4.10⁻⁴]的发射光谱较窄。这表明dGuo-C8-AAF和ro-dGuo-C8-AF I + II不会像dGuo-C8-AF那样衰变为激基复合物。根据各种化合物动态结构的差异对光谱特征进行了讨论。