Liepelt U, Kindler-Röhrborn A, Lennartz K, Reinhardt-Maelicke S, Rajewsky M F
Institut für Zellbiologie (Tumorforschung), Universität Essen, F.R.G.
Brain Res Dev Brain Res. 1990 Feb 1;51(2):267-78. doi: 10.1016/0165-3806(90)90285-7.
We have studied the differentiation potential in vitro of a subpopulation of neural progenitor cells from BDIX-rat brain. These cells transiently express a cell surface determinant (CSD) specified by monoclonal antibody (Mab) RB13-2 (Kindler-Röhrborn, A. et al., Differentiation 30 (1985) 53-60), and recently identified as a set of O-acetylated gangliosides (Reinhardt-Maelicke, S. et al., submitted) also recognized by Mab D1.1 (Levine, J.M. et al., J. Neurosci., 4 (1984) 826-831) and partly by Mab JONES (Schlosshauer, B. et al., J. Neurosci., 8 (1988) 580-592), respectively. As analyzed by immunofluorescence, Mab RB13-2 binding brain cells (prenatal days 11-22; postnatal days 7 and 89) were localized in different areas of the proliferative ventricular layer of the prenatal cerebrum and in the external granular layer of the early postnatal cerebellum. No Mab RB13-2 positive brain cells were found in adult brain. Following their isolation by fluorescence activated cell sorting on prenatal day 18, the differentiation potential of Mab RB13-2 binding brain cells was studied by double-immunofluorescence analysis under different conditions of monolayer culture. In the presence of 10% fetal calf serum (FCS), these cells differentiated into glial fibrillary acidic protein (GFAP) positive flat astrocytes, whereas neurons (neurofilament-positive) and a smaller number of stellate astrocytes (GFAP-positive) developed in a chemically defined medium containing 0.5% FCS. Neural progenitor cells binding Mab RB13-2 may thus either retain more than one option for differentiation into specific cell types, or the expression of the CSD specified by Mab RB13-2 may be common to more than one subset of neural progenitor cells (with or without predetermined unidirectional differentiation pathways) whose survival and/or proliferative behavior could be differentially affected by microenvironmental conditions.
我们研究了来自BDIX大鼠脑的神经祖细胞亚群在体外的分化潜能。这些细胞短暂表达一种由单克隆抗体（Mab）RB13-2所确定的细胞表面决定簇（CSD）（Kindler-Röhrborn, A.等人，《分化》30(1985)53 - 60），最近被鉴定为一组O-乙酰化神经节苷脂（Reinhardt-Maelicke, S.等人，已提交），Mab D1.1（Levine, J.M.等人，《神经科学杂志》，4(1984)826 - 831）也能识别，并且分别部分地被Mab JONES（Schlosshauer, B.等人，《神经科学杂志》，8(1988)580 - 592）识别。通过免疫荧光分析，与Mab RB13-2结合的脑细胞（产前第11 - 22天；产后第7天和第&9天）定位于产前大脑增殖性室管膜层的不同区域以及产后早期小脑的外颗粒层。在成体脑中未发现Mab RB13-2阳性的脑细胞。在产前第18天通过荧光激活细胞分选将它们分离后，通过在不同单层培养条件下的双重免疫荧光分析研究了与Mab RB13-2结合的脑细胞的分化潜能。在含有10%胎牛血清（FCS）时，这些细胞分化为胶质纤维酸性蛋白（GFAP）阳性的扁平星形胶质细胞，而在含有0.5%FCS的化学限定培养基中则发育出神经元（神经丝阳性）和数量较少的星状星形胶质细胞（GFAP阳性）。因此，与Mab RB13-2结合的神经祖细胞可能要么保留了向特定细胞类型分化的多种选择，要么Mab RB13-2所确定的CSD的表达可能在不止一个神经祖细胞亚群中常见（无论有无预定的单向分化途径），其存活和/或增殖行为可能受到微环境条件的不同影响。
