Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Appl Spectrosc. 2012 Dec;66(12):1403-10. doi: 10.1366/11-06566.
Laser-induced fluorescence (LIF) and intrinsic fluorescence spectroscopy (IFS) have been used experimentally for diagnosing coronary atherosclerosis. In this study, we demonstrated the diagnostic superiority of IFS at 342-nm excitation (IFS(342)) versus LIF (LIF(342)) and described a protocol for head-to-head comparison of old (LIF) versus new (IFS) generations of similar diagnostic methods, labeled as "generational comparison model". IFS(342) and LIF(342) were modeled with basis spectra of media, fibrous caps, and superficial foam cells and of their correspondent chemicals (elastin, collagen, and lipoproteins). The average accuracy and receiver operating characteristic area under the curve of IFS(342) in single-, double-, and triple-parameter diagnostic algorithm iterations, geared toward identifying 84 atherosclerotic specimens from a group of 117 coronary segments, was 90% ± 1% and 0.87 ± 0.025, superior to LIF(342) (84% ± 3% and 0.84 ± 0.016; P = 0.0002 and 0.02, respectively) in a generational comparison model.
激光诱导荧光（LIF）和固有荧光光谱（IFS）已被用于诊断冠状动脉粥样硬化的实验。在这项研究中，我们证明了在 342nm 激发下的 IFS（IFS(342)）比 LIF（LIF(342)）具有诊断优势，并描述了一种用于对头对头比较新旧（LIF）与类似诊断方法的新一代（IFS）的协议，称为“代际比较模型”。IFS(342)和 LIF(342)的模型建立基于介质、纤维帽和浅表泡沫细胞及其相应化学物质（弹性蛋白、胶原蛋白和脂蛋白）的基础光谱。在针对从 117 个冠状动脉段的一组中识别 84 个动脉粥样硬化标本的单参数、双参数和三参数诊断算法迭代中，IFS(342)的平均准确性和接收者操作特征曲线下面积分别为 90%±1%和 0.87±0.025，优于 LIF(342)（84%±3%和 0.84±0.016；P=0.0002 和 0.02，分别）在代际比较模型中。
