Bainbridge B W, Spreadbury C L, Scalise F G, Cohen J
Division of Biosphere Sciences, King's College, Kensington Campus, London, U.K.
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):113-7. doi: 10.1016/0378-1097(90)90267-t.
Improved methods are described for the isolation of pure, high molecular weight DNA from small and large scale cultures of filamentous fungi. The methods depend on the extraction of DNA under conditions which prevent nuclease activity and contamination by carbohydrate. The small scale method depends on enzymatic digestion of the wall whereas the large scale method uses partial damage followed by autolysis. High yields of DNA are obtained by both methods and the DNA is suitable for restriction analysis. Southern Blotting, RFLP analysis, dot blotting and the production of gene libraries. The small scale method can be used for the simultaneous analysis of multiple cultures.
本文描述了从丝状真菌的小规模和大规模培养物中分离纯的高分子量DNA的改进方法。这些方法依赖于在防止核酸酶活性和碳水化合物污染的条件下提取DNA。小规模方法依赖于细胞壁的酶促消化，而大规模方法则是先进行部分损伤然后自溶。两种方法都能获得高产率的DNA，且所得DNA适用于限制性分析、Southern印迹、RFLP分析、斑点印迹以及构建基因文库。小规模方法可用于同时分析多个培养物。
