Gould-Kostka J L, Burns D L, Brennan M J, Manclark C R
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, MD 20892.
FEMS Microbiol Lett. 1990 Feb;55(3):285-9. doi: 10.1016/0378-1097(90)90010-n.
A purification scheme was devised for a 69-kDa outer membrane protein of Bordetella pertussis, a virulence-associated protein which may play a role in the pathogenesis of the organism. The protein was purified to apparent homogeneity by heating B. pertussis cells for 1 h at 60 degrees C followed by DEAE-Sepharose and Affi-Gel Blue chromatography. Antibodies found in sera obtained from patients diagnosed as having pertussis reacted with this protein. This purification scheme should be useful for the production of the 69 kDa protein which is currently being evaluated as a pertussis vaccine candidate.
设计了一种针对百日咳博德特氏菌69 kDa外膜蛋白的纯化方案，该蛋白是一种与毒力相关的蛋白，可能在该生物体的发病机制中起作用。通过将百日咳博德特氏菌细胞在60℃加热1小时，然后进行DEAE-琼脂糖和Affi-Gel Blue层析，将该蛋白纯化至表观均一。从被诊断患有百日咳的患者血清中发现的抗体与该蛋白发生反应。这种纯化方案对于生产目前正在评估作为百日咳疫苗候选物的69 kDa蛋白应该是有用的。
