Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
Arch Microbiol. 2013 Feb;195(2):121-30. doi: 10.1007/s00203-012-0857-3. Epub 2012 Dec 13.
Enterococcus (E.) faecalis is found as commensal in healthy humans, in a variety of fermented foods. It can serve as probiotic but also as pathogen causing endocarditis, bacteremia and urinary tract infections. We have employed a proteomic study with E. faecalis strain OG1RF under different growth conditions and in contact to mouse intestinal cells to identify novel latent and adaptive fitness determinants. These relate to changes in catabolic pathways (BudA), protein biosynthesis (AsnS), cellular surface biosynthesis (RmlA) and regulatory mechanisms (OmpR). This knowledge can be used to derive novel evidence-based targets, which can be used to further elucidate gene expression changes enhancing pathogenicity or fitness in a commensal strain and possibly delineate this species into groups of higher and lower risk for applications in a food or a medical context versus improved treatment strategies of the so far hard to cure diseases.
肠球菌（E.）粪肠球菌存在于健康人体的共生体中，存在于各种发酵食品中。它可以作为益生菌，但也可以作为病原体，引起心内膜炎、菌血症和尿路感染。我们采用了一种蛋白质组学研究方法，研究了不同生长条件下和与小鼠肠道细胞接触的 OG1RF 粪肠球菌菌株，以鉴定新的潜在和适应性适应因子。这些与代谢途径（BudA）、蛋白质生物合成（AsnS）、细胞表面生物合成（RmlA）和调节机制（OmpR）的变化有关。这些知识可用于得出新的基于证据的靶点，这可以用于进一步阐明增强致病性或适应性的基因表达变化，使共生菌株具有更高或更低的风险，并可能将该物种划分为更高级和更低级的风险组，以便在食品或医学背景下应用，或改进目前难以治愈的疾病的治疗策略。
